| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R4 |
| Uniprot No | Q15257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-358aa |
| 氨基酸序列 | AEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFEYRVSEMWNEVHEEKEQAAKQSVSCDECIPLPRAGHCAPSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG |
| 预测分子量 | 60.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP2R4重组蛋白的参考文献示例(注：文献为假设性描述，实际引用需核实)：
1. **"Recombinant PPP2R4 Regulates PP2A Activity and Cell Cycle Progression"**
**作者**: Smith, J. et al.
**摘要**: 研究报道了重组PPP2R4蛋白在大肠杆菌中的表达与纯化，证实其通过调节PP2A磷酸酶活性影响细胞周期检查点，为理解PP2A在细胞周期调控中的作用提供依据。
2. **"Structural Insights into the PP2A Regulatory Subunit PPP2R4"**
**作者**: Johnson, R. et al.
**摘要**: 利用重组PPP2R4蛋白进行X射线晶体学分析，揭示了其与PP2A核心酶(催化亚基和支架亚基)的相互作用位点，为靶向PP2A的药物设计提供结构基础。
3. **"PPP2R4 Facilitates the Assembly of the PP2A Holoenzyme via Recombinant Protein Interaction Studies"**
**作者**: Lee, H. et al.
**摘要**: 通过体外重组蛋白共表达实验，证明PPP2R4作为分子伴侣促进PP2A全酶组装，并解析了其结合PP2A核心复合物的动力学特征。
4. **"Functional Characterization of Recombinant PPP2R4 in Cancer Cell Signaling"**
**作者**: Zhang, Y. et al.
**摘要**: 利用重组PPP2R4蛋白进行功能研究，发现其通过增强PP2A对AKT信号通路的去磷酸化作用，抑制肿瘤细胞增殖，提示其潜在抗癌治疗价值。
**提示**：实际研究中，PPP2R4可能以别名(如PP2A调节亚基B''亚家族成员)出现，建议结合UniProt(ID: Q15208)及PubMed最新文献进一步检索。
**Background of PPP2R4 Recombinant Protein**
PPP2R4. also known as PR53 or Regulatory Subunit B'' of Protein Phosphatase 2A (PP2A), is a critical component of the PP2A holoenzyme complex. PP2A is a ubiquitously expressed serine/threonine phosphatase involved in regulating diverse cellular processes, including cell cycle progression, signal transduction, and apoptosis. The PP2A holoenzyme typically consists of a structural scaffold subunit (A), a catalytic subunit (C), and a variable regulatory subunit (B-type), which determines substrate specificity and subcellular localization. PPP2R4 belongs to the B'' family of regulatory subunits and plays a role in directing PP2A activity toward distinct substrates.
Recombinant PPP2R4 protein is produced using expression systems (e.g., *E. coli* or mammalian cells*) to enable studies on its structure, function, and interactions. It is often engineered with tags (e.g., His-tag) for purification and detection. Research highlights its involvement in modulating PP2A’s dephosphorylation activity, particularly in pathways linked to cell survival, DNA damage response, and metabolic regulation. Dysregulation of PPP2R4 has been implicated in diseases such as cancer, where PP2A often acts as a tumor suppressor, and neurodegenerative disorders.
Studies using recombinant PPP2R4 have advanced understanding of PP2A complex assembly, substrate recruitment, and its crosstalk with kinases like AKT and mTOR. Its role in maintaining cellular homeostasis underscores its potential as a therapeutic target or biomarker in pathological conditions.
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