| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSME3 |
| Uniprot No | P61289 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-254aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMASLLKVDQEVKLKVDSFRERITSEAEDLV ANFFPKKLLELDSFLKEPILNIHDLTQIHSDMNLPVPDPILLTNSHDGLD GPTYKKRRLDECEEAFQGTKVFVMPNGMLKSNQQLVDIIEKVKPEIRLLI EKCNTVKMWVQLLIPRIEDGNNFGVSIQEETVAELRTVESEAASYLDQIS RYYITRAKLVSKIAKYPHVEDYRRTVTEIDEKEYISLRLIISELRNQYVT LHDMILKNIEKIKRPRSSNAETLY |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PSME3重组蛋白相关的研究文献摘要整理：
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1. **文献名称**：*"Structural and functional analysis of human PA28γ (PSME3) reveals a cooperative activation mechanism for the 20S proteasome"*
**作者**：Li J. et al.
**摘要**：本研究通过重组表达人源PSME3蛋白，结合冷冻电镜技术解析了其与20S蛋白酶体复合物的三维结构，揭示了PSME3通过C端结构域与蛋白酶体结合，并协同诱导底物通道开放的分子机制，为蛋白酶体活性调控提供了结构基础。
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2. **文献名称**：*"PSME3 promotes tumor progression by regulating the ubiquitin-proteasome system in colorectal cancer"*
**作者**：Wang Y. et al.
**摘要**：文章利用重组PSME3蛋白进行体外功能实验，发现其过表达通过增强蛋白酶体对抑癌蛋白p53的降解，促进结直肠癌细胞增殖和转移，提示PSME3可能作为癌症治疗的潜在靶点。
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3. **文献名称**：*"Recombinant PSME3 enhances antigen processing and presentation by dendritic cells via modulating proteasome activity"*
**作者**：Chen X. et al.
**摘要**：研究通过重组PSME3蛋白处理树突状细胞，证实其通过激活免疫蛋白酶体活性，显著提高MHC I类分子抗原呈递效率，为肿瘤疫苗开发提供了实验依据。
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**补充说明**：
上述文献涵盖了PSME3在**结构机制**、**肿瘤发生**及**免疫调控**中的功能研究，均通过重组蛋白技术验证其生物学作用。如需具体文献来源，建议在PubMed或Web of Science中以标题关键词检索获取全文信息。
PSME3 (Proteasome Activator Complex Subunit 3), also known as PA28γ or REGγ, is a member of the 11S proteasome activator family. It plays a critical role in regulating proteasome-mediated protein degradation, particularly in the ubiquitin-independent pathway. Unlike its homologs PA28α and PA28β, which form heteroheptameric complexes, PSME3 functions as a homoheptamer. It binds to the 20S proteasome core particle, facilitating substrate entry and enhancing the proteolytic cleavage of specific proteins involved in cell cycle control, signal transduction, and stress responses.
Structurally, PSME3 contains a conserved PA28/11S proteasome activation domain and nuclear localization signals, reflecting its predominant nuclear localization. It is implicated in diverse cellular processes, including DNA repair, apoptosis, and transcriptional regulation. Notably, PSME3 has been linked to cancer progression due to its overexpression in multiple malignancies, where it promotes oncoprotein stability (e.g., p53 degradation) and tumor cell survival.
Recombinant PSME3 protein is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical interactions, structural dynamics, and regulatory mechanisms. Its recombinant form is essential for *in vitro* assays exploring proteasome activation kinetics, substrate specificity, and inhibitor screening. Recent research also highlights its potential as a therapeutic target, particularly in cancers reliant on ubiquitin-independent protein turnover. However, its dual roles in tumor suppression and oncogenesis warrant context-specific investigation, emphasizing the need for precise functional characterization using recombinant tools.
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