| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIO2 |
| Uniprot No | Q92813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 166-265aa |
| 氨基酸序列 | PSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRM DNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKR |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DIO2(Ⅱ型脱碘酶)重组蛋白的虚构参考文献示例(仅供格式参考)：
1. **"Expression and Purification of Recombinant Human DIO2 in Escherichia coli"**
- 作者：Smith A, et al.
- 摘要：研究通过大肠杆菌表达系统成功制备重组人DIO2蛋白，优化了纯化条件，并验证其酶活性可将T4转化为T3.
2. **"Functional Characterization of Recombinant Mouse DIO2 in Mammalian Cells"**
- 作者：Zhang L, et al.
- 摘要：构建小鼠DIO2重组蛋白的哺乳动物表达载体，在HEK293细胞中表达并证实其温度敏感性及在甲状腺激素代谢中的调控作用。
3. **"Role of Recombinant DIO2 in Metabolic Disorders: In Vitro Analysis"**
- 作者：Brown K, et al.
- 摘要：利用重组DIO2蛋白探究其在肥胖和糖尿病模型中的功能，发现其活性变化与能量代谢异常相关。
4. **"Crystal Structure of Recombinant DIO2 Reveals Substrate Binding Sites"**
- 作者：Tanaka Y, et al.
- 摘要：首次解析重组DIO2蛋白的晶体结构，阐明其催化机制，为靶向药物设计提供结构基础。
注：以上文献为示例，实际引用请参考真实数据库(如PubMed)中的论文。
**Background of DIO2 Recombinant Protein**
Deiodinase iodothyronine type 2 (DIO2) is a selenocysteine-containing enzyme critical in regulating thyroid hormone metabolism. It catalyzes the outer-ring deiodination of the prohormone thyroxine (T4) to bioactive triiodothyronine (T3), a key regulator of energy homeostasis, growth, and development. DIO2 is primarily expressed in tissues such as the brain, pituitary, thyroid, and brown adipose tissue, playing a pivotal role in local T3 availability and adaptive thermogenesis.
Recombinant DIO2 protein is engineered using molecular cloning techniques, often expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to preserve enzymatic activity. Its production enables detailed study of DIO2 structure-function relationships, substrate specificity, and regulatory mechanisms (e.g., post-translational modifications, ubiquitination). Researchers utilize recombinant DIO2 to investigate thyroid-related disorders, including hypothyroidism, resistance to thyroid hormone (RTH), and metabolic syndromes linked to altered T3 signaling.
DIO2 dysfunction is implicated in conditions like metabolic obesity, neurodevelopmental defects, and mood disorders, making its recombinant form valuable for drug screening and therapeutic targeting. Studies also explore DIO2's role in adaptive responses to cold, fasting, and illness, where localized T3 production modulates tissue-specific metabolic demands.
The recombinant protein typically includes affinity tags (e.g., His-tag) for purification and detection. Activity assays often measure iodine release from T4 or T3 generation via spectrophotometric/radioactive methods. Challenges in production include maintaining selenocysteine incorporation (critical for catalytic activity) and avoiding oxidative inactivation.
Overall, recombinant DIO2 serves as a vital tool for dissecting thyroid hormone dynamics, developing diagnostic assays, and exploring therapeutic interventions for endocrine and metabolic diseases.
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