| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APPBP2 |
| Uniprot No | Q92624 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585aa |
| 氨基酸序列 | MAAVELEWIP ETLYNTAISA VVDNYIRSRR DIRSLPENIQ FDVYYKLYQQ GRLCQLGSEF CELEVFAKVL RALDKRHLLH HCFQALMDHG VKVASVLAYS FSRRCSYIAE SDAAVKEKAI QVGFVLGGFL SDAGWYSDAE KVFLSCLQLC TLHDEMLHWF RAVECCVRLL HVRNGNCKYH LGEETFKLAQ TYMDKLSKHG QQANKAALYG ELCALLFAKS HYDEAYKWCI EAMKEITAGL PVKVVVDVLR QASKACVVKR EFKKAEQLIK HAVYLARDHF GSKHPKYSDT LLDYGFYLLN VDNICQSVAI YQAALDIRQS VFGGKNIHVA TAHEDLAYSS YVHQYSSGKF DNALFHAERA IGIITHILPE DHLLLASSKR VKALILEEIA IDCHNKETEQ RLLQEAHDLH LSSLQLAKKA FGEFNVQTAK HYGNLGRLYQ SMRKFKEAEE MHIKAIQIKE QLLGQEDYEV ALSVGHLASL YNYDMNQYEN AEKLYLRSIA IGKKLFGEGY SGLEYDYRGL IKLYNSIGNY EKVFEYHNVL SNWNRLRDRQ YSVTDALEDV STSPQSTEEV VQSFLISQNV EGPSC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的 **APPBP2重组蛋白** 相关参考文献示例(非真实文献，仅供格式参考)：
---
1. **文献名称**: *APPBP2 regulates β-amyloid precursor protein processing through a novel caspase-3-dependent mechanism*
**作者**: Zhang Y, Chen L, et al.
**摘要**: 本研究利用重组APPBP2蛋白，发现其通过与β-淀粉样前体蛋白(APP)结合，激活Caspase-3通路，促进APP的异常切割，导致阿尔茨海默病相关Aβ肽段积累。
2. **文献名称**: *Structural characterization of recombinant APPBP2 and its role in neuronal differentiation*
**作者**: Kumar S, Lee JH, et al.
**摘要**: 通过大肠杆菌表达系统获得高纯度重组APPBP2蛋白，结合X射线晶体学解析其三维结构，并证明其在体外促进神经元细胞分化中的关键作用。
3. **文献名称**: *APPBP2 interacts with PTEN to modulate cell proliferation and apoptosis in cancer cells*
**作者**: Wang X, Li M, et al.
**摘要**: 重组APPBP2蛋白与PTEN的磷酸酶结构域直接结合，抑制PI3K/AKT信号通路，显著降低肿瘤细胞增殖并诱导凋亡，提示其潜在抗癌靶点价值。
4. **文献名称**: *Recombinant APPBP2 as a biomarker for early Alzheimer’s disease diagnosis*
**作者**: Gonzalez R, Patel K, et al.
**摘要**: 开发基于重组APPBP2蛋白的ELISA检测技术，发现其在阿尔茨海默病患者脑脊液中表达异常升高，或可作为早期诊断标志物。
---
**备注**：以上文献为示例性质，具体研究需参考真实数据库(如PubMed、Google Scholar)。建议用关键词 **"APPBP2 recombinant protein"** 或 **"APPBP2 AND (structure OR function)"** 检索最新文献。
**Background of APPBP2 Recombinant Protein**
APPBP2 (Amyloid Beta Precursor Protein Binding Protein 2), also known as PAT1 or NEDD8-interacting protein, is a multifunctional adaptor protein involved in diverse cellular processes. It was initially identified through its interaction with the amyloid precursor protein (APP), a key player in Alzheimer’s disease pathogenesis. Structurally, APPBP2 contains an N-terminal ubiquitin-like (UBL) domain and a C-terminal SH3-binding motif, enabling interactions with components of the ubiquitin-proteasome system and signaling pathways.
The protein participates in regulating protein degradation, cell cycle progression, and transcriptional activity. Notably, APPBP2 binds to NEDD8. a ubiquitin-like modifier, and facilitates neddylation—a post-translational modification critical for E3 ligase activation. This interaction links APPBP2 to the DNA damage response and cell survival mechanisms. Additionally, APPBP2 interacts with the Wnt/β-catenin and Notch signaling pathways, suggesting roles in development and oncogenesis.
Recombinant APPBP2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and functional roles *in vitro*. Its purified form enables structural analysis, enzymatic assays, and exploration of APP processing mechanisms. Researchers also utilize it to investigate APPBP2's involvement in neurodegenerative diseases, particularly its potential modulation of APP cleavage and Aβ peptide generation.
Beyond neurodegeneration, APPBP2 recombinant protein aids in cancer research, as aberrant expression correlates with tumor progression. Its role in neddylation and protein turnover makes it a candidate for therapeutic targeting. Overall, APPBP2 serves as a versatile tool for unraveling molecular pathways in disease and development.
×
