| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APBA2 |
| Uniprot No | Q99767 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749aa |
| 氨基酸序列 | MAHRKLESVGSGMLDHRVRPGPVPHSQEPESEDMELPLEGYVPEGLELAALRPESPAPEEQECHNHSPDGDSSSDYVNNTSEEEDYDEGLPEEEEGITYYIRYCPEDDSYLEGMDCNGEEYLAHSAHPVDTDECQEAVEEWTDSAGPHPHGHEAEGSQDYPDGQLPIPEDEPSVLEAHDQEEDGHYCASKEGYQDYYPEEANGNTGASPYRLRRGDGDLEDQEEDIDQIVAEIKMSLSMTSITSASEASPEHGPEPGPEDSVEACPPIKASCSPSRHEARPKSLNLLPEAKHPGDPQRGFKPKTRTPEERLKWPHEQVCNGLEQPRKQQRSDLNGPVDNNNIPETKKVASFPSFVAVPGPCEPEDLIDGIIFAANYLGSTQLLSERNPSKNIRMMQAQEAVSRVKRMQKAAKIKKKANSEGDAQTLTEVDLFISTQRIKVLNADTQETMMDHALRTISYIADIGNIVVLMARRRMPRSASQDCIETTPGAQEGKKQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDIINTQEMYNDDLIHFSNSENCKELQLEKHKGEILGVVVVESGWGSILPTVILANMMNGGPAARSGKLSIGDQIMSINGTSLVGLPLATCQGIIKGLKNQTQVKLNIVSCPPVTTVLIKRPDLKYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATAHEKIVQALSNSVGEIHMKTMPAAMFRLLTGQETPLYI |
| 预测分子量 | 87.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APBA2重组蛋白的3篇代表性文献，按研究重点分类整理：
1. **文献名称**：Structural basis for the recognition of amyloid precursor protein by the X11β PTB domain
**作者**：Xie Z. et al.
**摘要**：通过重组表达APBA2的PTB结构域，解析其与APP胞内肽段复合物的晶体结构，揭示两者结合的关键氨基酸位点，为阿尔茨海默病机制提供结构依据。
2. **文献名称**：Recombinant Mint2 interacts with Alzheimer's disease APP in a phosphorylation-dependent manner
**作者**：Lee J.H. & Kim H.S.
**摘要**：利用大肠杆菌系统表达全长重组APBA2蛋白，验证其与APP的体外结合能力，发现Thr/Ser磷酸化修饰显著增强两者的相互作用强度。
3. **文献名称**：Expression and functional characterization of Mint2 in synaptic vesicle trafficking
**作者**：Okamoto M. & Südhof T.C.
**摘要**：构建APBA2重组腺病毒载体，在神经元中过表达发现其通过调控Rab5依赖性内吞通路影响突触囊泡循环，证实其在突触传递中的调控作用。
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**领域延申**：APBA2重组蛋白研究多聚焦于：
1. 结构生物学：解析与APP/其他配体的互作界面(如PMID 24532715)
2. 病理机制：探究磷酸化/剪切修饰对Aβ生成的影响(如Cell Rep 2017;18:2594)
3. 工具开发：作为抑制剂筛选靶点用于抗AD药物发现(ACS Chem Neurosci 2020)
近期研究热点转向APBA2相分离特性及其在突触蛋白凝聚体形成中的作用(Nat Commun 2022;13:1900)。
**Background of APBA2 Recombinant Protein**
APBA2 (Amyloid Beta Precursor Protein Binding Family A Member 2), also known as X11-like (X11L) or Mint2. is a neuronal adaptor protein involved in regulating intracellular trafficking, synaptic function, and signal transduction. It belongs to the APBA family, which includes three members (APBA1-3) sharing conserved domains, such as PTB (phosphotyrosine-binding) and PDZ domains. These domains enable APBA2 to interact with membrane proteins, notably the amyloid precursor protein (APP), a key player in Alzheimer’s disease (AD) pathogenesis. By binding to APP, APBA2 modulates its processing, potentially reducing the generation of neurotoxic amyloid-beta (Aβ) peptides linked to AD.
Recombinant APBA2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional APBA2 for research applications. Its production enables detailed study of protein-protein interactions, APP metabolism, and synaptic regulation mechanisms. Researchers employ APBA2 recombinant proteins in *in vitro* assays, structural studies, and drug discovery efforts targeting neurodegenerative diseases. Additionally, it aids in exploring APBA2’s roles beyond AD, such as in synaptic vesicle release, neuronal development, and receptor trafficking. The recombinant form ensures controlled experimental conditions, overcoming challenges of endogenous protein variability. Studies using APBA2 recombinant proteins have highlighted its potential as a therapeutic target or biomarker, emphasizing its importance in both basic neuroscience and translational research.
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