| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APBA3 |
| Uniprot No | O96018 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-138aa |
| 氨基酸序列 | MDFPTISRSPSGPPAMDLEGPRDILVPSEDLTPDSQWDPMPGGPGSLSRM ELDESSLQELVQQFEALPGDLVGPSPGGAPCPLHIATGHGLASQEIADAH GLLSAEAGRDDLLGLLHCEECPPSQTGPEEPLEPAPRL |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APBA3重组蛋白的示例性参考文献(注：文献信息为示例性概括，具体内容建议通过学术数据库核实)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of APBA3 in Amyloid-β Pathway Regulation"
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组APBA3蛋白，并验证其与淀粉样前体蛋白(APP)的PTB结构域结合，揭示了APBA3在调节APP加工和β-分泌酶活性中的作用。
2. **文献名称**: "Mint3/APBA3 Recombinant Protein Enhances Tumor Metastasis via HIF-1 Signaling Activation"
**作者**: Yamamoto K, et al.
**摘要**: 利用哺乳动物细胞表达纯化的重组Mint3(APBA3)蛋白，证明其通过稳定HIF-1α促进肿瘤细胞糖代谢和侵袭能力，为癌症治疗提供了潜在靶点。
3. **文献名称**: "Structural Analysis of APBA3 PTB Domain in Complex with APP Peptide"
**作者**: Chen R, et al.
**摘要**: 通过重组表达APBA3的PTB结构域，结合X射线晶体学解析其与APP胞内肽段的复合物结构，阐明了二者特异性结合的分子机制。
4. **文献名称**: "Development of APBA3-Based Recombinant Protein Assay for Alzheimer’s Drug Screening"
**作者**: Gupta S, et al.
**摘要**: 构建了基于重组APBA3蛋白的高通量筛选平台，用于筛选调控APP-APBA3相互作用的小分子抑制剂，为阿尔茨海默病药物开发提供新策略。
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**说明**：以上文献为基于APBA3常见研究方向的概括性示例，实际文献需通过PubMed、Web of Science等平台以关键词“APBA3 recombinant”“Mint3 expression”检索获取。
APBA3 (Amyloid Beta Precursor Protein Binding Family A Member 3), also known as Mint3 or Neuron-specific X11-like protein, is a member of the X11/Mint protein family that interacts with amyloid precursor protein (APP) and other transmembrane proteins. It functions as an adaptor protein involved in intracellular trafficking, signal transduction, and protein complex stabilization. Structurally, APBA3 contains a phosphotyrosine-binding (PTB) domain that mediates interactions with the cytoplasmic domain of APP, as well as two PDZ domains for binding to additional partners.
Research on APBA3 has highlighted its role in regulating APP processing, potentially influencing amyloid-beta (Aβ) peptide production, a hallmark of Alzheimer’s disease pathology. Unlike its homologs APBA1 and APBA2. which are predominantly expressed in neurons, APBA3 exhibits broader tissue distribution, including non-neuronal cells like cancer cells and macrophages. This versatility links APBA3 to diverse physiological and pathological processes. For instance, studies show APBA3 promotes cancer metastasis by stabilizing hypoxia-inducible factor-1α (HIF-1α) to enhance glycolytic metabolism under low-oxygen conditions. It also modulates immune responses by regulating NF-κB signaling in macrophages.
Recombinant APBA3 proteins are engineered for in vitro studies to dissect its molecular interactions, structural motifs, and functional mechanisms. These proteins are typically expressed in bacterial or mammalian systems, purified via affinity chromatography, and validated for binding assays, enzymatic activity studies, or structural analysis. As a research tool, recombinant APBA3 aids in exploring therapeutic targets for neurodegenerative diseases, cancer, and inflammatory disorders. Its multifunctional nature underscores its importance in bridging cellular pathways, making it a subject of ongoing interdisciplinary investigation.
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