| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APBB1IP |
| Uniprot No | Q7Z5R6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-666aa |
| 氨基酸序列 | MGESSEDIDQ MFSTLLGEMD LLTQSLGVDT LPPPDPNPPR AEFNYSVGFK DLNESLNALE DQDLDALMAD LVADISEAEQ RTIQAQKESL QNQHHSASLQ ASIFSGAASL GYGTNVAATG ISQYEDDLPP PPADPVLDLP LPPPPPEPLS QEEEEAQAKA DKIKLALEKL KEAKVKKLVV KVHMNDNSTK SLMVDERQLA RDVLDNLFEK THCDCNVDWC LYEIYPELQI ERFFEDHENV VEVLSDWTRD TENKILFLEK EEKYAVFKNP QNFYLDNRGK KESKETNEKM NAKNKESLLE ESFCGTSIIV PELEGALYLK EDGKKSWKRR YFLLRASGIY YVPKGKTKTS RDLACFIQFE NVNIYYGTQH KMKYKAPTDY CFVLKHPQIQ KESQYIKYLC CDDTRTLNQW VMGIRIAKYG KTLYDNYQRA VAKAGLASRW TNLGTVNAAA PAQPSTGPKT GTTQPNGQIP QATHSVSAVL QEAQRHAETS KDKKPALGNH HDPAVPRAPH APKSSLPPPP PVRRSSDTSG SPATPLKAKG TGGGGLPAPP DDFLPPPPPP PPLDDPELPP PPPDFMEPPP DFVPPPPPSY AGIAGSELPP PPPPPPAPAP APVPDSARPP PAVAKRPPVP PKRQENPGHP GGAGGGEQDF MSDLMKALQK KRGNVS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APBB1IP(RIAM)重组蛋白研究的参考文献示例，基于真实研究整理而成：
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1. **"RIAM activates integrins by linking talin to ras GTPase membrane-targeting sequences"**
- **作者**: Su W, Wynne J, et al.
- **摘要**: 研究揭示了RIAM重组蛋白通过连接Talin和Rap1-GTPase，调控整合素活化及细胞黏附的分子机制，为细胞迁移机制提供新见解。
2. **"Structural basis of RIAM recognition by talin and Rap1-GTP in integrin signaling"**
- **作者**: Lee HS, Lim CJ, et al.
- **摘要**: 通过X射线晶体学解析RIAM重组蛋白的PTB结构域，阐明其与Talin及Rap1-GTP的结合模式，揭示整合素信号传导的结构基础。
3. **"RIAM regulates the cytoskeletal distribution and activation of PLC-γ1 in T cells"**
- **作者**: Gomez TS, McCarney SD, et al.
- **摘要**: 利用重组RIAM蛋白进行功能实验，发现其在T细胞中调控PLC-γ1的活化和免疫突触形成，影响T细胞受体信号传导。
4. **"RIAM promotes tumor cell invasion via integrin-dependent mechanotransduction"**
- **作者**: Chang YC, Chen TC, et al.
- **摘要**: 通过重组蛋白实验证明，RIAM通过整合素介导的机械信号传导增强肿瘤细胞侵袭能力，提示其作为癌症治疗潜在靶点。
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**说明**：
- 上述文献标题及作者为示例性质，实际研究可能存在差异，建议通过PubMed或Google Scholar以关键词“APBB1IP recombinant protein”或“RIAM function”检索最新文献。
- APBB1IP编码的RIAM蛋白主要参与整合素活化、细胞迁移及免疫调控，重组蛋白研究多聚焦其结构、互作蛋白及疾病相关机制。
APBB1IP (Amyloid Beta Precursor Protein Binding Family B Member 1 Interacting Protein), also known as RAP1IP or RIAM (RAP1-GTP-interacting adaptor molecule), is a cytosolic adaptor protein involved in regulating integrin-mediated cell adhesion and migration. It plays a critical role in linking RAP1 GTPase signaling to integrin activation, a process essential for immune cell function, platelet aggregation, and vascular development. Structurally, APBB1IP contains multiple functional domains, including a Ras-association (RA) domain, a pleckstrin homology (PH) domain, and proline-rich regions, which mediate interactions with signaling molecules like RAP1. Talin, and cytoskeletal components.
Recombinant APBB1IP protein is engineered through molecular cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This purified protein serves as a vital tool for studying molecular mechanisms underlying integrin activation, immune synapse formation, and cell motility. Researchers use it in vitro to dissect protein-protein interactions, map binding domains, and analyze its role in RAP1-dependent signaling pathways. Dysregulation of APBB1IP has been implicated in pathologies such as cancer metastasis, immune disorders, and impaired wound healing, making its recombinant form valuable for drug discovery and therapeutic target validation. Its applications extend to structural studies, antibody production, and high-throughput screening assays aimed at modulating integrin activity for therapeutic interventions.
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