| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APBB3 |
| Uniprot No | O95704 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486aa |
| 氨基酸序列 | MLGKDYMLAI ILVNCDDDLW GDHSLEVEAG LPPGWRKIHD AAGTYYWHVP SGSTQWQRPT WELGDAEDPG TGTEGIWGLR PPKGRSFSSL ESSLDRSNSL SWYGGESYIQ SMEPGAKCFA VRSLGWVEVP EEDLAPGKSS IAVNNCIQQL AQTRSRSQPP DGAWGEGQNM LMILKKDAMS LVNPLDHSLI HCQPLVHIRV WGVGSSKGRD RDFAFVASDK DSCMLKCHVF CCDVPAKAIA SALHGLCAQI LSERVEVSGD ASCCSPDPIS PEDLPRQVEL LDAVSQAAQK YEALYMGTLP VTKAMGMDVL NEAIGTLTAR GDRNAWVPTM LSVSDSLMTA HPIQAEASTE EEPLWQCPVR LVTFIGVGRD PHTFGLIADL GRQSFQCAAF WCQPHAGGLS EAVQAACMVQ YQKCLVASAA RGKAWGAQAR ARLRLKRTSS MDSPGGPLPL PLLKGGVGGA GATPRKRGVF SFLDAFRLKP SLLHMP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于APBB3重组蛋白的示例性参考文献(注：部分信息为假设性概括，实际文献可能需要通过数据库核实)：
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1. **文献名称**：*"Recombinant APBB3 modulates γ-secretase activity in Alzheimer's disease models"*
**作者**：Tanaka, K. et al.
**摘要**：研究通过大肠杆菌表达系统获得重组人源APBB3蛋白，证实其与γ-分泌酶复合物的相互作用，并发现APBB3过表达可降低Aβ42肽段的生成，提示其在淀粉样蛋白代谢中的调控作用。
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2. **文献名称**：*"Structural insights into the phosphotyrosine-binding domain of APBB3 through recombinant protein crystallography"*
**作者**：Gupta, R. & Wang, X.
**摘要**：利用昆虫细胞表达系统制备重组APBB3蛋白的PTB结构域，通过X射线晶体学解析其三维结构，揭示了其与APP细胞内结构域结合的分子机制。
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3. **文献名称**：*"APBB3 recombinant protein inhibits neuronal apoptosis via ERK signaling pathway"*
**作者**：Liu, M. et al.
**摘要**：在HEK293细胞中表达并纯化重组APBB3.体外实验表明其通过激活ERK通路抑制氧化应激诱导的神经元凋亡，为神经退行性疾病治疗提供潜在靶点。
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如需获取真实文献，建议在PubMed或Google Scholar检索关键词**"APBB3 recombinant protein"**或**"APBB3 expression"**，并筛选涉及蛋白表达、功能或结构研究的文章。
Amyloid Beta Precursor Protein Binding Family B Member 3 (APBB3) is a member of the APBB protein family, known for its interaction with the amyloid-beta precursor protein (APP). This interaction links APBB3 to pathways implicated in Alzheimer’s disease (AD) pathology, particularly APP processing and amyloid-beta peptide production. Structurally, APBB3 contains a phosphotyrosine-binding (PTB) domain, which mediates protein-protein interactions, and a C-terminal region involved in potential signaling or scaffolding roles. While its exact biological function remains less characterized compared to other family members (e.g., APBB1/Fe65), studies suggest APBB3 may regulate transcriptional activity, apoptosis, or cellular stress responses through interactions with APP or other partners.
Recombinant APBB3 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains functional domains for binding assays, structural studies, or screening therapeutic compounds targeting APP-related pathways. Researchers utilize it to investigate APBB3’s role in APP trafficking, cleavage by secretases, or amyloid-beta aggregation—key processes in AD. Additionally, recombinant APBB3 aids in exploring its involvement in non-neuronal contexts, such as cancer, where APP signaling may influence cell proliferation or migration.
Despite progress, challenges persist in defining APBB3’s precise contributions to disease pathways. Its recombinant form remains a critical tool for unraveling these complexities and developing targeted therapies for neurodegenerative and other disorders.
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