| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIF |
| Uniprot No | O95831 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 121-613aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEEVPQDKAPSHVPFLLIGGGTAAFAAARS IRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGK ERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGS QITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISRE VKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLS NWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVG LEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGR RRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVD SSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPA VPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHE DLNEVAKLFNIHED |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AIF(凋亡诱导因子)重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Molecular characterization of mitochondrial apoptosis-inducing factor*
**作者**：Susin, S. A., et al.
**摘要**：该研究首次克隆并表达了重组AIF蛋白，证明其在体外能诱导染色体凝缩和DNA断裂，揭示了AIF依赖caspase非依赖的细胞凋亡机制。
2. **文献名称**：*Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death*
**作者**：Joza, N., et al.
**摘要**：通过基因敲除小鼠模型，发现AIF重组蛋白在胚胎发育中调控caspase非依赖性凋亡，缺乏AIF导致神经细胞异常存活及胚胎致死。
3. **文献名称**：*Interaction between AIF and CHCHD4 regulates respiratory chain biogenesis*
**作者**：Hangen, E., et al.
**摘要**：研究利用重组AIF蛋白揭示其与线粒体膜蛋白CHCHD4的互作，阐明AIF在维持呼吸链复合物组装及细胞能量代谢中的双重功能。
这些文献涵盖了AIF重组蛋白在凋亡机制、发育生物学及代谢调控中的关键作用，为相关研究提供了理论及技术基础。
Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein with dual roles in cellular homeostasis and apoptosis. Initially identified for its capacity to trigger caspase-independent cell death, AIF translocates to the nucleus under apoptotic stimuli, inducing chromatin condensation and DNA fragmentation. Beyond its pro-apoptotic function, AIF is essential for maintaining mitochondrial integrity, energy metabolism, and redox balance through its NADH oxidase activity. Genetic mutations or depletion of AIF are linked to severe neurodegenerative disorders, such as Parkinson's disease, and developmental defects, highlighting its physiological significance.
Recombinant AIF proteins, produced via heterologous expression systems like *E. coli* or mammalian cells, are pivotal tools for studying structure-function relationships and disease mechanisms. These proteins retain key functional domains, including the FAD-binding module critical for redox activity and the C-terminal region necessary for nuclear localization. Researchers employ recombinant AIF to investigate its interaction with DNA, chromatin-modifying enzymes, and mitochondrial partners like heat shock proteins. Additionally, recombinant variants are explored for therapeutic potential, such as mitigating neuronal death in stroke models or enhancing cancer cell sensitivity to chemotherapy. However, challenges persist in mimicking post-translational modifications (e.g., phosphorylation) that regulate AIF's dual roles. Current studies also focus on engineering AIF mutants to dissect its apoptotic versus pro-survival signals, offering insights for targeted therapies in neurodegeneration and ischemia-reperfusion injuries.
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