| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAWP |
| Uniprot No | Q6ICG8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MAVNQSHTENRRGALIPNGESLLKRSPNVELSFPQRSEGSNVFSGRKTGTLFLTSYRVIFITSCSISDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFRNGDAIEFAQLMVKAASAAARGFPLRTLNDWFSSMGIYVITGEGNMCTPQMPCSVIVYGAPPAGYGAPPPGYGAPPAGYGAQPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASPAGSGARPQESTAAQAPENEASLPSASSSQVHS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAWP重组蛋白的3篇参考文献示例(注：以下为虚构文献，仅作参考)：
---
1. **文献名称**：*PAWP Recombinant Protein Induces Oocyte Activation in Mammalian Fertilization*
**作者**：Sutovsky P., et al.
**摘要**：研究证实重组PAWP蛋白能够模拟精子进入卵母细胞后的激活作用，通过触发钙振荡促进胚胎发育，为辅助生殖技术提供新策略。
---
2. **文献名称**：*Expression and Functional Characterization of Human PAWP in Sperm Maturation*
**作者**：Aarabi M., et al.
**摘要**：通过大肠杆菌系统成功表达重组PAWP蛋白，揭示其在精子顶体反应和穿透卵子透明带中的关键功能，为男性不育机制研究提供依据。
---
3. **文献名称**：*PAWP as a Diagnostic Marker for Sperm Quality Assessment*
**作者**：Yanagimachi R., et al.
**摘要**：利用重组PAWP蛋白开发新型精子质量检测方法，发现PAWP表达水平与精子受精能力正相关，可用于临床男性生育力评估。
---
(如需真实文献，建议通过PubMed或Google Scholar检索关键词“PAWP recombinant protein”或“PAWP fertilization”。)
**Background of PAWP Recombinant Protein**
PAWP (Post-Acrosomal WW Domain-Containing Protein), also known as WBP2NL, is a sperm-specific protein implicated in oocyte activation during fertilization. Initially identified in 2007. PAWP is localized to the post-acrosomal sheath of sperm and shares structural homology with PLCζ, another sperm-derived oocyte-activating factor. Its name derives from the presence of a WW domain, a protein-protein interaction module, and its phospholipid-binding properties.
PAWP is proposed to function as a molecular trigger for initiating calcium oscillations in the oocyte, a critical event for embryonic development. Studies suggest that PAWP interacts with oocyte-specific proteins like YAP1 (Yes-associated protein 1) or components of the PI3K/Akt pathway, potentially modulating signaling cascades post-sperm-egg fusion. However, its exact mechanism remains debated, with some research highlighting its sufficiency to induce oocyte activation, while others emphasize its auxiliary role alongside PLCζ.
Recombinant PAWP is produced using expression systems like *E. coli* or mammalian cells, enabling in vitro studies of its biochemical properties and functional validation. Its applications span basic research in reproductive biology, diagnostics for male infertility (e.g., cases linked to sperm activation deficiencies), and developing therapeutic strategies for assisted reproductive technologies (ART), such as improving intracytoplasmic sperm injection (ICSI) outcomes.
Despite its potential, controversies persist regarding PAWP's physiological necessity, urging further mechanistic studies. Nonetheless, PAWP remains a focal point in understanding fertilization and advancing treatments for infertility.
×
