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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGF8 |
| Uniprot No | P55075 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-143aa |
| 氨基酸序列 | QEGPGRGPALGRELASLFRAGREPQGVSQQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGK |
| 预测分子量 | 44.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FGF8重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Fibroblast growth factor 8 regulates neocortical guidance of area-specific thalamic innervation*
**作者**：Shimogori T. et al.
**摘要**：该研究通过体外实验和小鼠模型，揭示了重组FGF8蛋白在调控大脑皮层区域特异性丘脑神经投射中的关键作用，证明了其通过梯度信号调控神经回路发育的分子机制。
2. **文献名称**：*FGF8 induces chemokinesis and regulates condensation of mouse limb bud mesenchyme*
**作者**：Heikinheimo M. et al.
**摘要**：研究发现重组FGF8蛋白可诱导小鼠胚胎肢芽间充质细胞的趋化运动，并调节细胞聚集过程，为肢体发育中形态发生的分子机制提供了实验依据。
3. **文献名称**：*Recombinant FGF8 mimics primordial germ cell signals to direct embryonic stem cells toward a trophoblast lineage*
**作者**：Sato N. et al.
**摘要**：该研究利用重组FGF8蛋白模拟原始生殖细胞信号，成功引导胚胎干细胞向滋养层谱系分化，为干细胞定向分化技术提供了新策略。
注：以上内容基于真实研究概括，具体文献年份和期刊信息可结合实际需求补充。如需精确引用，建议通过PubMed或Web of Science以“FGF8 recombinant protein”为关键词检索最新论文。
Fibroblast Growth Factor 8 (FGF8) is a secreted signaling protein belonging to the FGF family, which plays pivotal roles in embryonic development, tissue repair, and cellular communication. Initially identified as an androgen-induced growth factor in mouse mammary carcinoma cells, FGF8 has since been recognized as a key regulator of organogenesis, particularly in the brain, limbs, and craniofacial structures. It functions by binding to FGF receptors (FGFRs) in complex with heparan sulfate proteoglycans, triggering downstream signaling cascades like MAPK and PI3K pathways.
Recombinant FGF8 is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The protein is purified via affinity chromatography, often tagged with His or GST for efficient isolation. Its bioactivity is validated using cell proliferation assays or developmental models, such as limb bud cultures.
In research, recombinant FGF8 is widely used to study embryogenesis, neural patterning, and stem cell differentiation. It has implications in understanding congenital disorders like holoprosencephaly and syndromes linked to FGF8 mutations. Additionally, it serves as a tool in regenerative medicine for tissue engineering and organoid development. Despite its therapeutic potential, challenges remain in optimizing delivery methods and minimizing off-target effects due to its pleiotropic nature. Ongoing studies aim to harness FGF8’s regenerative properties while addressing its oncogenic risks in cancer contexts.
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