| 经过测试的应用 | |
| WB | WB-1/500 - 1/2000 |
| IF/ICC | IF/ICC-1/200 - 1/1000 |
| FCM | FCM-1/200 - 1/400 |
| 免疫原 | Purified recombinant fragment of human SIGLEC8 expressed in E. Coli. |
| 反应物种 | Human,Mouse,Monkey,Rat |
| 抗体类型 | Primary antibody |
| 克隆性 | Monoclonal |
| 基因名 | SIGLEC8 |
| 形态 | Liquid |
| 浓度 | 1ug/ul |
| 分子量 | 54kDa |
| 形式 | Liquid |
| 存储缓冲液 | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
| 储存 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Western blot analysis using SIGLEC8 mouse mAb against Hela (1), MCF-7 (2), Jurkat (3), T47D (4), K562 (5), A431 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunofluorescence analysis of 3T3-L1 cells using SIGLEC8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH/3T3 cells using SIGLEC8 mouse mAb (blue) and negative control (red).
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