| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRF1 |
| Uniprot No | P14222 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-555aa |
| 氨基酸序列 | PCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW |
| 预测分子量 | 103.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRF1(Perforin-1)重组蛋白的模拟参考文献示例，涵盖其表达、功能及疾病关联研究：
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1. **文献名称**：*Recombinant Expression and Structural Characterization of Human Perforin-1 in Escherichia coli*
**作者**：Chen L, et al.
**摘要**：本研究通过大肠杆菌系统成功表达了重组人源PRF1蛋白，并利用X射线晶体学解析了其跨膜结构域的构象，揭示了其与靶细胞膜相互作用的分子机制，为后续功能研究奠定了基础。
2. **文献名称**：*Functional Analysis of PRF1 Mutants Using Recombinant Protein in Cytotoxic Lymphocyte Models*
**作者**：Voskoboinik I, et al.
**摘要**：通过哺乳动物细胞系统表达重组PRF1及其常见致病突变体(如A91V、H222P)，发现突变体无法形成功能性跨膜孔道，阐明了PRF1缺陷导致家族性噬血细胞综合征(FHL)的分子机制。
3. **文献名称**：*Recombinant PRF1-Mediated Pore Formation in Lipid Bilayers: A Single-Molecule Study*
**作者**：Praper T, et al.
**摘要**：利用重组PRF1蛋白在人工脂质双层中进行单分子成像，揭示了其在靶膜上寡聚化形成孔道的动态过程，定量分析了孔道大小与钙离子浓度的依赖性。
4. **文献名称**：*Development of a Cell-Based Assay for PRF1 Activity Screening Using Recombinant Protein*
**作者**：Brennan AJ, et al.
**摘要**：构建基于重组PRF1蛋白的细胞毒性检测平台，结合荧光标记靶细胞，评估了PRF1在NK细胞杀伤中的功能，并验证了其作为免疫治疗靶点的潜力。
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**注**：以上文献为示例性内容，实际引用需通过PubMed或专业数据库检索真实发表的研究。研究关键词建议：*"recombinant perforin-1"*, *"PRF1 expression"*, *"pore-forming protein"*。
**Background of PRF1 Recombinant Protein**
Perforin-1 (PRF1), encoded by the *PRF1* gene, is a cytotoxic effector protein predominantly expressed in natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). It plays a critical role in immune defense by forming pores in the membranes of target cells, facilitating the entry of pro-apoptotic granzymes to induce programmed cell death. This mechanism is essential for eliminating virus-infected cells, tumor cells, and regulating immune homeostasis.
Recombinant PRF1 protein is engineered using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to produce a purified, functional form of the protein for research and therapeutic applications. The recombinant protein retains the key structural features of native PRF1. including a membrane-attack complex/perforin (MACPF) domain critical for pore formation and a calcium-binding site necessary for its activity.
Studies involving PRF1 recombinant protein have advanced understanding of immune dysregulation disorders. Mutations in *PRF1* are linked to familial hemophagocytic lymphohistiocytosis (FHL type 2), a severe immune deficiency characterized by defective cytotoxic granule-mediated apoptosis. Recombinant PRF1 enables functional assays to dissect these mutations' effects, aiding in diagnostics and targeted therapies.
Additionally, recombinant PRF1 is used in cancer immunotherapy research to enhance cytotoxic lymphocyte activity or engineer chimeric antigen receptor (CAR) T cells. Its role in autoimmune and inflammatory diseases is also under exploration, as dysregulated perforin activity may contribute to tissue damage.
In summary, PRF1 recombinant protein serves as a vital tool for elucidating immune cytotoxicity mechanisms, modeling diseases, and developing novel immunotherapies. Its applications bridge basic immunology and clinical translation, addressing gaps in treating immune disorders and malignancies.
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