| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCT |
| Uniprot No | P09683 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-54aa |
| 氨基酸序列 | HSD GTFTSELSRL REGARLQRLL QGLV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于SCT(Secretin)重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: "High-Yield Expression and Purification of Recombinant Human Secretin in Escherichia coli"
**作者**: Zhang et al.
**摘要**: 研究通过优化大肠杆菌表达系统，实现了人源SCT重组蛋白的高效表达与纯化，并通过质谱和体外活性实验验证了其结构与生物活性，为大规模生产奠定基础。
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2. **文献名称**: "Therapeutic Potential of Recombinant Secretin in a Rat Model of Chronic Pancreatitis"
**作者**: Wang et al.
**摘要**: 探讨重组SCT蛋白对慢性胰腺炎模型大鼠的治疗效果，结果显示其可显著减轻炎症反应并改善胰腺外分泌功能，提示其临床转化潜力。
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3. **文献名称**: "Structural and Functional Characterization of a Novel Secretin Variant Produced in Pichia pastoris"
**作者**: Lee et al.
**摘要**: 利用毕赤酵母系统表达新型SCT重组蛋白变体，通过晶体结构解析和受体结合实验揭示其与天然激素相似的构效关系，为药物开发提供新思路。
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注：以上文献为示例，实际引用时需检索PubMed、Web of Science等数据库获取真实研究数据。
**Background of SpyCatcher/SpyTag (SCT) Recombinant Proteins**
SpyCatcher/SpyTag (SCT) technology is a breakthrough in protein engineering, enabling precise, covalent linkage of protein modules through spontaneous isopeptide bond formation. Developed in 2012 by Zakeri and Howarth, the system harnesses a natural protein interaction derived from the *Streptococcus pyogenes* adhesin Spy0125. SpyCatcher is a 15 kDa protein domain that binds irreversibly to SpyTag, a short 13-residue peptide, forming a covalent bond between lysine and aspartate residues. This reaction occurs rapidly under mild conditions (e.g., neutral pH, room temperature), without requiring additional enzymes or chemical crosslinkers.
The SCT system revolutionized modular protein assembly by offering unparalleled specificity and efficiency. Traditional methods for conjugating proteins often rely on stochastic chemical modifications or complex genetic fusions, which can compromise functionality or yield. In contrast, SCT enables precise "plug-and-play" assembly of multifunctional protein complexes, nanomachines, or biomaterials. Its applications span diverse fields:
- **Vaccine development**: SpyCatcher-SpyTag fusions allow antigen display on virus-like particles (VLPs) or nanoparticles, enhancing immune responses (e.g., COVID-19 vaccine candidates).
- **Enzyme engineering**: Modular assembly of enzyme cascades improves catalytic efficiency.
- **Drug delivery**: Functionalization of carriers with targeting ligands or therapeutics.
- **Diagnostics**: Immobilization of detection probes on biosensors.
- **Synthetic biology**: Construction of artificial cellular machinery.
The technology’s versatility is further expanded by orthogonal "Catcher-Tag" pairs (e.g., SnoopCatcher/SnoopTag), enabling multi-component assemblies. Its robustness in vitro and in vivo, compatibility with standard expression systems (e.g., *E. coli*), and scalability make SCT a cornerstone of modern bioengineering, accelerating innovations in therapeutics, biotechnology, and materials science.
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