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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCP2 |
| Uniprot No | P80075 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-99aa |
| 氨基酸序列 | QPDSVSIPITCCFNVINRKIPIQRLESYTRITNIQCPKEAVIFKTKRGKE VCADPKERWVRDSMKHLDQIFQNLKP |
| 预测分子量 | 9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCP2重组蛋白的参考文献示例(注：以下内容为假设性示例，实际文献需通过学术数据库验证)：
1. **"High-yield Expression and Purification of Recombinant MCP2 in Escherichia coli"**
*Authors: Zhang L. et al.*
**摘要**: 本研究优化了在大肠杆菌系统中表达和纯化MCP2重组蛋白的方法，通过亲和层析和尺寸排阻色谱获得高纯度蛋白，并验证其结构完整性，为后续功能研究奠定基础。
2. **"Immunogenicity of Virus-like Particles Assembled from Recombinant MCP2 Protein"**
*Authors: Lee S. et al.*
**摘要**: 利用重组MCP2蛋白自组装形成病毒样颗粒(VLPs)，在小鼠模型中诱导强烈的体液和细胞免疫应答，表明其在疫苗开发中的潜在应用价值。
3. **"Development of an ELISA Diagnostic Kit Based on Recombinant MCP2 for Iridovirus Detection"**
*Authors: Wang Y. et al.*
**摘要**: 以重组MCP2蛋白作为抗原建立ELISA检测方法，用于快速筛查水产动物虹彩病毒感染，验证结果显示高灵敏度和特异性，优于传统PCR方法。
4. **"Structural Insights into the Receptor-binding Mechanism of Recombinant MCP2 Protein"**
*Authors: Smith J. et al.*
**摘要**: 通过冷冻电镜解析重组MCP2蛋白的三维结构，揭示其与宿主细胞表面受体结合的分子机制，为抗病毒药物设计提供结构基础。
**注意**：以上为模拟示例，实际研究中MCP2可能指代不同蛋白(如病毒衣壳蛋白或趋化因子)，建议结合具体研究领域检索文献。
**Background of MCP2 Recombinant Protein**
MCP2 (Major Capsid Protein 2) is a structural protein encoded by certain viruses, most notably members of the *Polyomaviridae* family. It plays a critical role in viral assembly, genome packaging, and host cell entry. In polyomaviruses, MCP2 forms the outer capsid shell, providing stability and mediating interactions with cellular receptors. Its structural and functional significance has made it a target for virological research, particularly in understanding viral pathogenesis and developing antiviral strategies.
Recombinant MCP2 protein is produced using genetic engineering techniques, where the *MCP2* gene is cloned into expression vectors (e.g., bacterial, insect, or mammalian systems) and purified via chromatography. This approach allows large-scale production of the protein while retaining its native conformation and antigenicity.
Research on recombinant MCP2 has focused on its applications in vaccine development, diagnostic tools, and molecular studies. For instance, it serves as an antigen in serological assays to detect viral infections or evaluate immune responses. Structural studies using recombinant MCP2 have provided insights into capsid assembly mechanisms and potential sites for therapeutic intervention. Additionally, engineered MCP2 variants are explored as vectors for gene delivery due to their ability to self-assemble into virus-like particles (VLPs), which can encapsulate nucleic acids or drugs.
Recent advances include the use of recombinant MCP2 in nanotechnology and personalized medicine, leveraging its biocompatibility and targeting specificity. Challenges remain in optimizing expression yields, minimizing aggregation, and enhancing immunogenicity for clinical applications. Nonetheless, MCP2 recombinant protein continues to be a valuable tool in virology, immunology, and bioengineering.
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