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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCP3 |
| Uniprot No | P80098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-99aa |
| 氨基酸序列 | QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKE ICADPTQKWVQDFMKHLDKKTQTPKL |
| 预测分子量 | 9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCP3重组蛋白的模拟参考文献示例(仅供学术写作参考，建议通过PubMed或Google Scholar核实真实文献)：
1. **《Expression and Functional Characterization of Recombinant MCP3 in Inflammatory Models》**
- 作者：Smith J, et al.
- 摘要：研究通过大肠杆菌系统重组表达MCP3蛋白，验证其趋化单核细胞和T淋巴细胞的功能，并证明其在慢性炎症小鼠模型中增强免疫细胞募集的作用。
2. **《Structural Analysis of MCP3 Reveals Key Domains for Chemokine Receptor Binding》**
- 作者：Li X, Wang Y.
- 摘要：通过X射线晶体学解析MCP3重组蛋白的三维结构，确定其与CCR1/CCR2受体结合的关键氨基酸残基，为靶向药物设计提供依据。
3. **《Optimization of MCP3 Production in Mammalian Cell Culture Systems》**
- 作者：Garcia R, et al.
- 摘要：对比HEK293和CHO细胞表达MCP3的产量与糖基化修饰差异，优化培养条件以提高重组蛋白的稳定性和生物活性。
4. **《MCP3 Recombinant Protein Attenuates Fibrosis in Liver Injury Models》**
- 作者：Chen L, et al.
- 摘要：发现重组MCP3通过调控巨噬细胞极化减轻小鼠肝纤维化，提示其潜在治疗价值。
**注意**：以上文献为模拟内容，实际研究中请通过学术数据库检索真实文献(如搜索关键词“MCP3 recombinant protein”或“CCL7 expression”)。
MCP-3 (Monocyte Chemoattractant Protein-3), also known as CCL7. is a member of the CC chemokine family involved in immune regulation and inflammatory responses. It was initially identified for its ability to recruit monocytes, lymphocytes, and eosinophils to sites of inflammation or infection. Structurally, MCP-3 is a small, secreted protein (~11 kDa) characterized by conserved cysteine residues (CC motif) that form disulfide bonds critical for receptor binding. It signals through G protein-coupled receptors, primarily CCR1. CCR2. and CCR3. which are expressed on various immune cells, including monocytes, dendritic cells, and T cells.
Recombinant MCP-3 is produced using expression systems such as *E. coli* or mammalian cells, followed by purification techniques like affinity chromatography. This engineered protein retains the functional properties of native MCP-3. enabling its use in research to study chemokine-mediated processes. Its role in diseases has been widely explored: MCP-3 contributes to chronic inflammation in conditions like rheumatoid arthritis, atherosclerosis, and asthma by promoting leukocyte infiltration. In cancer, it exhibits dual roles—supporting tumor progression via angiogenesis and immune evasion, or suppressing metastasis by enhancing antitumor immunity.
Studies also highlight MCP-3's involvement in tissue repair and neuroinflammation, linking it to neurodegenerative diseases. Recombinant MCP-3 serves as a tool to investigate receptor interactions, cellular migration assays, and therapeutic target validation. Despite its pathogenic associations, MCP-3's pleiotropic effects underscore the complexity of chemokine networks, necessitating context-specific analysis. Ongoing research aims to harness its regulatory potential while mitigating off-target effects, positioning MCP-3 as a focal point in immunology and drug development.
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