| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF582 |
| Uniprot No | Q96NG8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-517 aa |
| 活性数据 | MSLGSELFRDVAIVFSQEEWQWLAPAQRDLYRDVMLETYSNLVSLGLAVSKPDVISFLEQGKEPWMVERVVSGGLCPVLESRYDTKELFPKQHVYEVESPQWEIMESLTSYGLECSSFQDDWECRNQFDRQQGNPDRHFHQMIIRHEEMPTFDQHASLTFYQKIHTREKPFGYNKCRKDFWQKELLINHQGIYTNEKPYKCKECGKAFKYGSRLIQHENIHSGKKPYECKECGKAFNSGSNFIQHQRVHTGEKPYECKDCEKAFSRSSQLIEHQRTHTGEKPYQCKECGKAFNRISHLKVHYRIHTGEKPYACKECGKTFSHRSQLIQHQTVHTGRKLYECKECGKAFNQGSTLIRHQRIHTGEKPYECKVCGKAFRVSSQLKQHQRIHTGEKPYQCKVCGRAFKRVSHLTVHYRIHTGEKPYECKECGKAFSHCSQLIHHQVIHTEKKPYEYKECEKTLSHDSTTVQPQRMHNRETHVNIINVEKPSISSYPLLIIREFMLASNHMNGSNGESPLA |
| 分子量 | 86.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人ZNF582蛋白及其功能研究的文献示例(内容为虚拟概括，实际文献需根据具体研究补充)：
1. **文献名称**：**"ZNF582 methylation as a potential biomarker for oral cancer detection"**
**作者**：Chen Y. et al.
**摘要**：研究报道了ZNF582基因启动子区甲基化水平在口腔鳞状细胞癌患者中的异常升高，通过构建重组ZNF582蛋白发现其可与特定DNA序列结合，抑制肿瘤相关基因的转录，提示其可能作为表观遗传沉默的靶点用于癌症诊断。
2. **文献名称**：**"Functional characterization of recombinant ZNF582 protein in cervical cancer progression"**
**作者**：Wang L. et al.
**摘要**：通过大肠杆菌系统表达重组ZNF582蛋白，发现其在宫颈癌细胞中通过调控TGF-β信号通路抑制上皮间质转化(EMT)，证实ZNF582具有抑制肿瘤转移的潜在功能。
3. **文献名称**：**"Structural analysis and DNA-binding properties of the zinc finger domain of ZNF582"**
**作者**：Zhang X. et al.
**摘要**：利用X射线晶体学解析了重组ZNF582蛋白锌指结构域的3D结构，揭示了其与靶DNA结合的特异性位点，为理解其在基因调控中的分子机制提供了结构基础。
注：实际研究中需根据具体论文补充真实信息，以上为示例性内容。
**Background of Recombinant Human ZNF582 Protein**
The recombinant human Zinc Finger Protein 582 (ZNF582) is a genetically engineered protein derived from the ZNF582 gene, which encodes a transcription factor belonging to the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. ZNF582 contains multiple C2H2-type zinc finger motifs, enabling sequence-specific DNA binding and regulation of gene expression. It plays roles in cellular processes such as differentiation, proliferation, and apoptosis, and is implicated in tumor suppression, particularly in cancers like cervical, lung, and esophageal cancers, where its promoter hypermethylation correlates with carcinogenesis.
Recombinant ZNF582 is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. Its production allows researchers to explore molecular mechanisms, such as epigenetic regulation or DNA damage responses, and evaluate its potential as a diagnostic biomarker or therapeutic target. Structural studies of the recombinant protein also aid in mapping DNA-binding domains or interaction interfaces with other cellular partners.
Research on ZNF582 highlights its dual role: while it may act as a tumor suppressor in certain contexts, dysregulation or epigenetic silencing has been linked to disease progression. Recombinant ZNF582 tools are pivotal in clarifying these paradoxes and advancing translational applications in oncology.
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