| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF577 |
| Uniprot No | Q9BSK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-478 aa |
| 活性数据 | MSVRREQGSSSGEGSLSFEDVAVGFTREEWQFLDQSQKVLYKEVMLENYINLVSIGYRGTKPDSLFKLEQGEPPGIVEGAAHSQICPGFVIQSRRYAGKDSDAFGGYGRSCLHIKCDKTLTGVKYHRCVKPSSPKSQLNDLQKICAGGKPHECSVCGRAFSRKAQLIQHQRTERGEKPHGCGECGKTFMRKIQLTEHQRTHTGEKPHECSECGKAFSRKSQLMVHQRTHTGEKPYRCSECGKAFSRKCRLNRHQRSHTGEKLYGCSVCGKAFSQKAYLTAHQRLHTGDKPYKCSDCGRTFYFKSDLTRHQRIHTGEKPYECSECEKAFRSKSKLIQHQRTHTGERPYSCRECGKAFAHMSVLIKHEKTHIRETAINSLTVEKPSSRSHTSLYMSELIQEQKTVNTVPIEMPSSGTPPLLNKSERLVGRNVVIVEQPFPRNQAFVVNQEFEQRISLTNEVNVAPSVINYILYLTDIVSE |
| 分子量 | 80.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF577蛋白的3篇参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Cloning and Expression of Recombinant Human ZNF577 in Escherichia coli"*
**作者**：Chen L. et al.
**摘要**：本研究成功克隆了ZNF577基因，并利用大肠杆菌表达系统高效表达重组ZNF577蛋白，通过亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**：*"ZNF577 Methylation Status as a Novel Biomarker in Gastric Cancer"*
**作者**：Wang Y. et al.
**摘要**：探讨了ZNF577启动子甲基化在胃癌中的作用，发现ZNF577在癌组织中高甲基化且表达下调，可能通过调控Wnt通路抑制肿瘤进展。
3. **文献名称**：*"Functional Characterization of Recombinant ZNF577 as a Transcriptional Repressor in Breast Cancer"*
**作者**：Kim S. et al.
**摘要**：通过体外实验证明重组ZNF577蛋白结合特定DNA序列并抑制癌基因转录，提示其作为抑癌因子在乳腺癌中的潜在作用。
4. **文献名称**：*"Structural Analysis of ZNF577 Reveals Zinc Finger Domain Interactions with DNA"*
**作者**：Martinez R. et al.
**摘要**：解析了重组ZNF577蛋白的晶体结构，揭示其锌指结构域与DNA双链结合的分子机制，为设计靶向药物提供结构基础。
建议通过PubMed、Web of Science等平台检索实际文献。
**Background of Recombinant Human ZNF577 Protein**
Zinc finger protein 577 (ZNF577), a member of the C2H2-type zinc finger protein family, is characterized by conserved tandem zinc finger motifs that enable DNA or RNA binding, suggesting roles in transcriptional regulation or chromatin remodeling. ZNF577 is predominantly expressed in epithelial tissues and has been implicated in cellular differentiation, proliferation, and tumor suppression.
Studies link ZNF577 to cancer biology, where its downregulation or epigenetic silencing through promoter hypermethylation is observed in multiple cancers, including breast, gastric, and colorectal cancers. This loss correlates with enhanced tumor progression and metastasis, highlighting its potential as a tumor suppressor. Mechanistically, ZNF577 may regulate oncogenic pathways by modulating gene expression networks involved in cell cycle control or apoptosis.
Recombinant human ZNF577 protein, produced using expression systems like *E. coli* or mammalian cells, retains functional domains for biochemical studies. It serves as a tool to investigate DNA-protein interactions, epigenetic regulation, and therapeutic targeting. Further research aims to unravel its precise molecular functions and clinical relevance in disease diagnostics or treatment strategies.
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