| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF574 |
| Uniprot No | Q6ZN55 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-896 aa |
| 活性数据 | MTEESEETVLYIEHRYVCSECNQLYGSLEEVLMHQNSHVPQQHFELVGVADPGVTVATDTASGTGLYQTLVQESQYQCLECGQLLMSPSQLLEHQELHLKMMAPQEAVPAEPSPKAPPLSSSTIHYECVDCKALFASQELWLNHRQTHLRATPTKAPAPVVLGSPVVLGPPVGQARVAVEHSYRKAEEGGEGATVPSAAATTTEVVTEVELLLYKCSECSQLFQLPADFLEHQATHFPAPVPESQEPALQQEVQASSPAEVPVSQPDPLPASDHSYELRNGEAIGRDRRGRRARRNNSGEAGGAATQELFCSACDQLFLSPHQLQQHLRSHREGVFKCPLCSRVFPSPSSLDQHLGDHSSESHFLCVDCGLAFGTEALLLAHRRAHTPNPLHSCPCGKTFVNLTKFLYHRRTHGVGGVPLPTTPVPPEEPVIGFPEPAPAETGEPEAPEPPVSEETSAGPAAPGTYRCLLCSREFGKALQLTRHQRFVHRLERRHKCSICGKMFKKKSHVRNHLRTHTGERPFPCPDCSKPFNSPANLARHRLTHTGERPYRCGDCGKAFTQSSTLRQHRLVHAQHFPYRCQECGVRFHRPYRLLMHRYHHTGEYPYKCRECPRSFLLRRLLEVHQLVVHAGRQPHRCPSCGAAFPSSLRLREHRCAAAAAQAPRRFECGTCGKKVGSAARLQAHEAAHAAAGPGEVLAKEPPAPRAPRATRAPVASPAALGSTATASPAAPARRRGLECSECKKLFSTETSLQVHRRIHTGERPYPCPDCGKAFRQSTHLKDHRRLHTGERPFACEVCGKAFAISMRLAEHRRIHTGERPYSCPDCGKSYRSFSNLWKHRKTHQQQHQAAVRQQLAEAEAAVGLAVMETAVEALPLVEAIEIYPLAEAEGVQISG |
| 分子量 | 125.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF574蛋白的3篇代表性文献摘要示例(注：文献为虚拟示例，实际研究中请核实具体论文)：
1. **"Functional Characterization of Recombinant Human ZNF574 Protein in Transcriptional Regulation"**
*Author: Zhang L, et al.*
摘要：本研究成功在大肠杆菌中表达了重组人ZNF574蛋白，并通过电泳迁移实验(EMSA)证实其能特异性结合富含GC的DNA序列，揭示了其在基因转录调控中的潜在作用。
2. **"Structural Analysis of ZNF574 Zinc Finger Domains by Recombinant Protein Expression"**
*Author: Kim S, et al.*
摘要：通过重组技术表达并纯化ZNF574的锌指结构域，结合X射线晶体学解析其三维结构，阐明了其与DNA相互作用的关键氨基酸残基。
3. **"Role of Recombinant ZNF574 in Cellular Differentiation"**
*Author: Patel R, et al.*
摘要：利用哺乳动物细胞系表达重组ZNF574.发现其过表达可抑制胚胎干细胞向神经前体细胞分化，提示其在细胞命运决定中的调控功能。
4. **"ZNF574 Knockdown and Recombinant Rescue in Cancer Cell Models"**
*Author: García M, et al.*
摘要：通过siRNA沉默ZNF574基因后，癌细胞增殖速率增加;外源添加重组ZNF574蛋白可部分恢复表型，表明其可能作为肿瘤抑制因子发挥作用。
提示：实际研究中建议通过PubMed或Google Scholar以“ZNF574 recombinant protein”“ZNF574 function”等关键词检索最新文献。锌指蛋白家族功能多样性较高，具体研究需结合实验背景分析。
Zinc finger protein 574 (ZNF574) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZNF) family, which represents one of the largest groups of transcription regulators in humans. These proteins typically feature tandem C2H2-type zinc finger motifs for sequence-specific DNA binding and an N-terminal KRAB domain that mediates interactions with co-repressors like KAP1/TRIM28. facilitating chromatin remodeling and transcriptional repression. ZNF574 is hypothesized to regulate gene expression by targeting specific DNA sequences, potentially influencing cellular processes such as differentiation, development, or retrotransposon silencing. While its exact biological roles remain unclear, studies suggest tissue-specific expression patterns, with higher levels detected in testes and brain tissues. Dysregulation of ZNF574 has been loosely associated with cancers and neurodevelopmental disorders, though mechanistic insights are limited. As a recombinant protein, ZNF574 is often utilized in molecular studies to explore its DNA-binding specificity, protein interaction networks, or transcriptional regulatory functions via techniques like ChIP-seq and luciferase reporter assays. Current research focuses on mapping its genomic targets, characterizing its interplay with epigenetic modifiers, and elucidating its physiological and pathological relevance. However, comprehensive functional data are still lacking, highlighting the need for further investigation.
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