| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BSDL |
| Uniprot No | B0BM89 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-552aa |
| 氨基酸序列 | MALWATLTLAMACLVVAHAKTLGVVHTEGGFVEGTNKKIGVVFPDYIDIFKGIPFAAPPKPFEKAEKHPGWSGTLQAKDYKPRCLQATITQDNVFGSLDCLYLNVWVPQTRSQVSTNLPVMVWIFGGGFLLGSGQGANFLSNYLYDGEEVAMRGKVIVVTFNYRVGPLGFLSTGDSNAPGNYGLWDQHMAIAWVKRNIAAFGGDPNNITLFGESAGGASVSLQTLTPYNVGLIKRAISQSGVGLCPWAIQRDPLTWAKSLASKLGCPVNNTKELADCLRNTDPGALTIAYQLQLFNLEYPLVHYLAYSPVIDGDFIPDEPRNLFSNAAGVDYIAGVNNMDGHLFAGVDLPAINQPLHKISTEQVQKVVRGLTLAKGVPALDIAYDLYTAGWGTNPSQEDMKRTVVEVETDYIFLVATQEALALHYQNAKGAKTYSYMFSHPSRMPVYPSWMGADHAEDLQFMFGKPFSTPLAYRPRDRDVAQYMIAYWTNFAATGDPSKGESSIPTPWLAYSTENGQYLEINHKINYQSMKQSLRSPYVRFWAQTFINLPNA |
| 预测分子量 | 60,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BSDL(胆盐依赖性脂肪酶)重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"High-level expression of recombinant human bile salt-dependent lipase in Pichia pastoris"*
**作者**: Petersen et al. (2005)
**摘要**: 研究利用毕赤酵母系统高效表达重组人BSDL，优化了发酵条件并验证了酶的活性与天然蛋白相似，适用于大规模生产。
2. **文献名称**: *"Crystal structure of bile salt-dependent lipase from human pancreas"*
**作者**: Rüdiger et al. (2010)
**摘要**: 通过X射线晶体学解析人源BSDL的三维结构，揭示了胆盐结合域和催化机制，为酶工程改造提供结构基础。
3. **文献名称**: *"Recombinant BSDL as a potential therapeutic enzyme for celiac disease"*
**作者**: Moreno et al. (2018)
**摘要**: 评估重组BSDL降解麸质毒性肽的能力，证明其在体外模拟消化中可有效减少免疫原性，提示治疗乳糜泻的潜力。
以上文献涵盖表达系统开发、结构解析及临床应用方向，可根据研究需求进一步查阅原文。
**Background of BSDL Recombinant Protein**
Bile Salt-Dependent Lipase (BSDL), also known as carboxyl ester lipase (CEL), is a digestive enzyme primarily secreted by the pancreas and plays a critical role in lipid metabolism. It hydrolyzes dietary lipids, including cholesterol esters, triglycerides, and fat-soluble vitamins, facilitating their absorption in the intestine. BSDL’s activity is uniquely dependent on bile salts, which activate its enzymatic function and stabilize its structure. Beyond digestion, BSDL has been implicated in non-digestive roles, such as modulating immune responses and interacting with cellular membranes, suggesting broader physiological significance.
The production of recombinant BSDL leverages genetic engineering to express the protein in heterologous systems, such as *E. coli*, yeast, or mammalian cells. Recombinant technology addresses limitations of native BSDL purification, such as low yield and contamination risks from biological sources. Key challenges include preserving BSDL’s functional tertiary structure and post-translational modifications (e.g., glycosylation), which are critical for its enzymatic activity. Mammalian expression systems, like CHO cells, are often preferred to ensure proper folding and glycosylation, mimicking the native protein’s properties.
Recombinant BSDL has applications in biomedical research, including studies on lipid metabolism disorders, pancreatitis, and cystic fibrosis. It also serves as a tool for investigating enzyme kinetics or lipid-protein interactions. Clinically, recombinant BSDL holds therapeutic potential for pancreatic insufficiency or enzyme replacement therapies. Ongoing research focuses on optimizing expression systems and engineering BSDL variants with enhanced stability or tailored substrate specificity, expanding its utility in both basic science and translational medicine.
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