| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF287 |
| Uniprot No | Q9HBT7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-754 aa |
| 活性数据 | MNSSSRSQIL LRWKSDKAQS GPYNVEKEIL TSRFLRDTET CRQNFRNFPY PDLAGPRKAL SQLRELCLKW LRPEIHSKEQ ILELLVLEQF LTILPGEVRT WVKSQYPESS EEAVTLVEDL TQILEEEAPQ NSTLSQDTPE EDPRGKHAFQ TGWLNDLVTK ESMTFKDVAV DITQEDWELM RPVQKELYKT VTLQNYWNMV SLGLTVYRPT VIPILEEPWM VIKEILEGPS PEWETKAQAC TPVEDMSKLT KEETHTIKLE DSYDYDDRLE RRGKGGFWKI HTDERGFSLK SVLSQEYDPT EECLSKYDIY RNNFEKHSNL IVQFDTQLDN KTSVYNEGRA TFNHVSYGIV HRKILPGEKP YKCNVCGKKF RKYPSLLKHQ STHAKEKSYE CEECGKEFRH ISSLIAHQRM HTGEKPYECH QCGKAFSQRA HLTIHQRIHT GEKPYKCDDC GKDFSQRAHL TIHQRTHTGE KPYKCLECGK TFSHSSSLIN HQRVHTGEKP YICNECGKTF SQSTHLLQHQ KIHTGKKPYK CNECWKVFSQ STYLIRHQRI HSGEKCYKCN ECGKAFAHSS TLIQHQTTHT GEKSYICNIC GKAFSQSANL TQHHRTHTGE KPYKCSVCGK AFSQSVHLTQ HQRIHNGEKP FKCNICGKAY RQGANLTQHQ RIHTGEKPYK CNECGKAFIY SSSLNQHQRT HTGERPYKCN ECDKDFSQRT CLIQHQRIHT GEKPYACRIC GKTFTQSTNL IQHQRVHTGA KHRN |
| 分子量 | 88.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF287蛋白的模拟参考文献示例(注：以下文献为虚构示例，仅供格式参考，实际文献需通过学术数据库检索)：
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1. **文献名称**: *ZNF287 as a novel oncogenic regulator in breast cancer via KRAS signaling*
**作者**: Liu, X. et al.
**摘要**: 本研究揭示了ZNF287通过结合KRAS启动子区增强其转录活性，促进乳腺癌细胞增殖和迁移。实验表明，ZNF287敲除可抑制肿瘤生长，提示其作为治疗靶点的潜力。
2. **文献名称**: *Structural analysis of human ZNF287 reveals atypical zinc finger motifs*
**作者**: Patel, R. & Kim, J.
**摘要**: 利用X射线晶体学解析了ZNF287蛋白的C端锌指结构域，发现其含有非典型Cys-His排列，可能影响DNA结合特异性。研究为理解其基因调控机制提供了结构基础。
3. **文献名称**: *ZNF287 mediates transcriptional repression through interaction with HDAC complexes*
**作者**: Zhang, Y. et al.
**摘要**: 发现ZNF287通过与组蛋白去乙酰化酶(HDAC1/2)复合物互作，抑制靶基因(如p21)表达。染色质免疫沉淀实验证实其在表观遗传调控中的作用。
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**说明**：实际研究中建议通过**PubMed/Google Scholar**检索关键词如“ZNF287 protein”“recombinant ZNF287”或“ZNF287 function”获取真实文献。如需特定论文全文，可提供DOI号或PMID进一步协助定位。
Zinc finger protein 287 (ZNF287), a member of the Krüppel-associated box zinc finger protein (KRAB-ZFP) family, is a transcription factor encoded by the ZNF287 gene in humans. KRAB-ZFPs constitute one of the largest families of DNA-binding proteins in mammals, typically characterized by tandem arrays of C2H2-type zinc finger motifs responsible for sequence-specific DNA recognition and a KRAB domain at the N-terminus that mediates interactions with co-repressors like KAP1 (TRIM28). These proteins play critical roles in transcriptional regulation, chromatin remodeling, and suppression of transposable elements. ZNF287 is proposed to function as a transcriptional repressor by recruiting KAP1. which facilitates heterochromatin formation through histone modification complexes (e.g., HP1. SETDB1), thereby silencing target genes or retrotransposons.
While ZNF287’s precise biological roles remain under investigation, emerging evidence links it to cell proliferation, differentiation, and tumorigenesis. It displays context-dependent dual roles in cancers—acting as an oncogene in some tissues (e.g., promoting hepatocellular carcinoma growth) or a tumor suppressor in others. Alternative splicing generates multiple isoforms, adding complexity to its functional characterization. Recombinant ZNF287 proteins, often engineered with tags (e.g., His, GFP), enable in vitro studies of its DNA-binding specificity, protein interactions, and regulatory mechanisms. Such research aids in deciphering its contributions to development, epigenetic silencing, and disease pathways, highlighting its potential as a therapeutic target or biomarker.
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