| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PROS |
| Uniprot No | P07225 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-676aa |
| 氨基酸序列 | ANSLLEETKQGNLERECIEELCNKEEAREVFENDPETDYFYPKYLVCLRSFQTGLFTAARQSTNAYPDLRSCVNAIPDQCSPLPCNEDGYMSCKDGKASFTCTCKPGWQGEKCEFDINECKDPSNINGGCSQICDNTPGSYHCSCKNGFVMLSNKKDCKDVDECSLKPSICGTAVCKNIPGDFECECPEGYRYNLKSKSCEDIDECSENMCAQLCVNYPGGYTCYCDGKKGFKLAQDQKSCEVVSVCLPLNLDTKYELLYLAEQFAGVVLYLKFRLPEISRFSAEFDFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS |
| 预测分子量 | 86.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PROS重组蛋白的示例参考文献(注：文献为虚构示例，实际文献需通过学术数据库查询)：
---
1. **文献名称**: "High-Yield Expression and Purification of PROS Recombinant Protein in E. coli"
**作者**: Smith J, et al.
**摘要**: 研究开发了一种通过大肠杆菌表达系统高效生产PROS重组蛋白的优化策略，通过密码子优化和诱导条件调整，实现高产量可溶性蛋白，并采用亲和层析技术纯化，为大规模制备奠定了基础。
2. **文献名称**: "Structural Insights into PROS Recombinant Protein by X-ray Crystallography"
**作者**: Tanaka H, et al.
**摘要**: 通过X射线晶体学解析了PROS重组蛋白的原子级结构，揭示了其与配体结合的活性位点，为基于结构的药物设计和功能机制研究提供了关键数据。
3. **文献名称**: "PROS Recombinant Protein Enhances Neuronal Regeneration in Vitro"
**作者**: Chen R, et al.
**摘要**: 体外实验表明，PROS重组蛋白能够显著促进神经元轴突再生，其作用机制可能与激活PI3K/AKT信号通路相关，提示其在神经退行性疾病治疗中的潜力。
4. **文献名称**: "Therapeutic Efficacy of PROS Recombinant Protein in a Murine Inflammatory Model"
**作者**: Müller S, et al.
**摘要**: 在小鼠炎症模型中，PROS重组蛋白通过抑制NF-κB通路减轻组织炎症反应，证明了其在治疗慢性炎症性疾病中的临床应用前景。
---
**提示**：实际研究中，建议通过PubMed、Web of Science或Google Scholar等平台，以关键词“PROS recombinant protein”或“PROS protein expression”检索最新文献。
PROS recombinant proteins, derived from the PROS1 gene encoding Protein S, are critical components in regulating coagulation and inflammatory responses. Protein S, a vitamin K-dependent glycoprotein, functions as a cofactor for activated protein C (APC) in degrading coagulation factors Va and VIIIa, thereby inhibiting excessive blood clotting. It also exhibits direct anticoagulant activity through interactions with tissue factor pathway inhibitor (TFPI) and possesses anti-inflammatory and cytoprotective properties via Tyro3/Axl/Mer (TAM) receptor signaling pathways.
Recombinant PROS proteins are produced using biotechnological platforms (e.g., mammalian, insect, or yeast expression systems) to overcome limitations of plasma-derived Protein S, including supply constraints and pathogen contamination risks. These engineered proteins often feature optimized post-translational modifications, particularly γ-carboxylation for membrane-binding functionality, achieved through advanced cell line engineering or hybrid expression strategies.
Therapeutic applications focus on addressing Protein S deficiency – linked to thrombotic disorders like venous thromboembolism – and modulating inflammatory conditions. Recent research explores their potential in cancer therapy, leveraging PROS proteins' ability to promote phagocytosis of apoptotic cells and mitigate tumor-associated inflammation. Challenges remain in replicating the full γ-carboxylation profile of native Protein S and scaling production while maintaining cost-effectiveness. Current developments include fusion proteins combining PROS domains with albumin or Fc regions to enhance pharmacokinetic properties. As molecular engineering techniques advance, PROS recombinant proteins are emerging as versatile biologics with applications extending beyond hematology into immunology and oncology.
×
