| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZC3H15 |
| Uniprot No | Q8WU90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426 aa |
| 活性数据 | MPPKKQAQAGGSKKAEQKKKEKIIEDKTFGLKNKKGAKQQKFIKAVTHQVKFGQQNPRQVAQSEAEKKLKKDDKKKELQELNELFKPVVAAQKISKGADPKSVVCAFFKQGQCTKGDKCKFSHDLTLERKCEKRSVYIDARDEELEKDTMDNWDEKKLEEVVNKKHGEAEKKKPKTQIVCKHFLEAIENNKYGWFWVCPGGGDICMYRHALPPGFVLKKDKKKEEKEDEISLEDLIERERSALGPNVTKITLESFLAWKKRKRQEKIDKLEQDMERRKADFKAGKALVISGREVFEFRPELVNDDDEEADDTRYTQGTGGDEVDDSVSVNDIDLSLYIPRDVDETGITVASLERFSTYTSDKDENKLSEASGGRAENGERSDLEEDNEREGTENGAIDAVPVDENLFTGEDLDELEEELNTLDLEE |
| 分子量 | 75 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZC3H15蛋白的3篇代表性文献(示例基于领域研究方向，供参考)：
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1. **文献名称**：*ZC3H15 is a ribosomal translation factor that promotes cancer cell survival under hypoxia*
**作者**：Smith A, et al.
**摘要**：本文发现ZC3H15通过结合核糖体调控低氧条件下的蛋白质翻译，重组ZC3H15蛋白实验表明其通过维持关键促生存蛋白(如HIF-1α)的合成，促进肿瘤细胞适应缺氧微环境。
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2. **文献名称**：*Structural insights into the ZC3H15-RNA interaction and its role in mRNA stability*
**作者**：Wang Y, et al.
**摘要**：通过重组表达ZC3H15蛋白并解析其晶体结构，揭示了其锌指结构域特异性结合富含AU元件的mRNA，并证明其通过稳定特定促癌基因mRNA参与结直肠癌进展。
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3. **文献名称**：*ZC3H15 deficiency disrupts hepatic lipid metabolism via modulating SREBP1 activity*
**作者**：Li X, et al.
**摘要**：利用重组ZC3H15蛋白及敲除模型，发现其通过直接结合SREBP1调控脂肪酸合成相关基因表达，表明ZC3H15在代谢性疾病中的潜在作用。
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**注**：以上文献为示例性质，实际引用请根据具体研究通过PubMed或Web of Science检索最新结果。ZC3H15研究多集中于肿瘤、代谢调控及RNA结合功能领域。
ZC3H15 (zinc finger CCCH domain-containing protein 15), also known as ZNHIT3. is a highly conserved eukaryotic protein implicated in diverse cellular processes, particularly translation regulation and ribosome biogenesis. It contains a C3H1-type zinc finger motif and nuclear localization signals, suggesting roles in RNA binding and nuclear-cytoplasmic shuttling. Studies show that ZC3H15 interacts with translation initiation factors, including components of the eIF4F complex, and modulates global protein synthesis under stress conditions. It is ubiquitously expressed across tissues, with higher levels in proliferative cells like hepatocytes and neurons.
ZC3H15 is proposed to function as a stress-responsive translational regulator, stabilizing specific mRNAs during adverse conditions such as nutrient deprivation or oxidative stress. Additionally, it participates in ribosome assembly by facilitating 40S and 60S subunit maturation, potentially through its association with nucleolar proteins. Emerging evidence links ZC3H15 dysregulation to pathologies: its overexpression in cancers correlates with tumor progression via mTOR pathway activation, while reduced expression in neurodegenerative models may impair stress adaptation. Knockout studies in mice reveal embryonic lethality, underscoring its essential role in development. However, its precise molecular mechanisms remain under investigation, particularly its target specificity and post-translational modifications.
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