| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB48 |
| Uniprot No | P10074 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-688 aa |
| 活性数据 | MDGSFVQHSVRVLQELNKQREKGQYCDATLDVGGLVFKAHWSVLACCSHFFQSLYGDGSGGSVVLPAGFAEIFGLLLDFFYTGHLALTSGNRDQVLLAARELRVPEAVELCQSFKPKTSVGQAAGGQSGLGPPASQNVNSHVKEPAGLEEEEVSRTLGLVPRDQEPRGSHSPQRPQLHSPAQSEGPSSLCGKLKQALKPCPLEDKKPEDCKVPPRPLEAEGAQLQGGSNEWEVVVQVEDDGDGDYMSEPEAVLTRRKSNVIRKPCAAEPALSAGSLAAEPAENRKGTAVPVECPTCHKKFLSKYYLKVHNRKHTGEKPFECPKCGKCYFRKENLLEHEARNCMNRSEQVFTCSVCQETFRRRMELRVHMVSHTGEMPYKCSSCSQQFMQKKDLQSHMIKLHGAPKPHACPTCAKCFLSRTELQLHEAFKHRGEKLFVCEECGHRASSRNGLQMHIKAKHRNERPHVCEFCSHAFTQKANLNMHLRTHTGEKPFQCHLCGKTFRTQASLDKHNRTHTGERPFSCEFCEQRFTEKGPLLRHVASRHQEGRPHFCQICGKTFKAVEQLRVHVRRHKGVRKFECTECGYKFTRQAHLRRHMEIHDRVENYNPRQRKLRNLIIEDEKMVVVALQPPAELEVGSAEVIVESLAQGGLASQLPGQRLCAEESFTGPGVLEPSLIITAAVPEDCDT |
| 分子量 | 103.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZBTB48蛋白的3篇代表性文献的简要概括(基于近年研究主题，可能存在虚拟调整)：
1. **文献名称**：*ZBTB48 is a telomere-associated protein and protects telomeres in human cells*
**作者**：Li J.S., et al.
**摘要**：研究揭示ZBTB48通过结合端粒重复序列参与端粒长度调控，利用重组ZBTB48蛋白证实其体外结合端粒DNA的能力，并揭示其在抑制端粒过度延伸中的作用。
2. **文献名称**：*Structural insights into the DNA-binding specificity of ZBTB48 zinc finger domain*
**作者**：Wang Y., Zhang L.
**摘要**：通过体外表达纯化重组人ZBTB48蛋白的锌指结构域，结合晶体结构分析与DNA互作实验，阐明其特异性识别端粒相关序列的分子机制。
3. **文献名称**：*Recombinant ZBTB48 protein inhibits cancer cell proliferation via telomere dysfunction*
**作者**：Chen H., et al.
**摘要**：研究利用重组ZBTB48蛋白处理多种癌细胞系，发现其通过干扰端粒保护蛋白复合物(shelterin)功能，诱导端粒损伤并抑制肿瘤细胞增殖。
注：以上文献为示例性描述，实际研究中建议通过PubMed或Web of Science以“ZBTB48”、“TZAP”、“recombinant protein”为关键词检索最新文献。
**Background of Recombinant Human ZBTB48 Protein**
ZBTB48. also known as human telomeric zinc finger-associated protein (TZAP), is a member of the zinc finger and BTB/POZ domain-containing protein family. It functions as a transcription factor involved in regulating telomere homeostasis, DNA damage response, and cellular senescence. ZBTB48 binds directly to telomeric repeats via its C-terminal zinc finger motifs, competing with shelterin components like TRF1/TRF2 to modulate telomere length and protect genomic stability.
Studies suggest ZBTB48 plays dual roles in cancer: it may act as a tumor suppressor by limiting excessive telomere elongation in stem cells or promoting apoptosis under genotoxic stress, but its overexpression has also been linked to poor prognosis in certain cancers. Additionally, ZBTB48 interacts with TP53 (p53) to regulate stress-induced transcriptional programs. Recombinant human ZBTB48 protein, typically produced in *E. coli* or mammalian expression systems, retains these functional domains, enabling researchers to study its DNA-binding properties, protein interactions, and regulatory mechanisms *in vitro*. Its structural features include an N-terminal BTB/POZ domain critical for protein dimerization and chromatin remodeling, and multiple zinc finger motifs for sequence-specific DNA recognition. Research on recombinant ZBTB48 provides insights into aging-related disorders, cancer biology, and potential therapeutic targeting of telomere-associated pathways.
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