| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB8 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441 aa |
| 活性数据 | MEISSHQSHLLQQLNEQRRQDVFCDCSILVEGKVFKAHRNVLFASSGYFKMLLSQNSKETSQPTTATFQAFSPDTFTVILDFVYSGKLSLTGQNVIEVMSAASFLQMTDVISVCKTFIKSSLDISEKEKDRYFSLSDKDANSNGVERSSFYSGGWQEGSSSPRSHLSPEQGTGIISGKSWNKYNYHPASQKNTQQPLAKHEPRKESIKKTKHLRLSQPSEVTHYKSSKREVRTSDSSSHVSQSEEQAQIDAEMDSTPVGYQYGQGSDVTSKSFPDDLPRMRFKCPYCTHVVKRKADLKRHLRCHTGERPYPCQACGKRFSRLDHLSSHFRTIHQACKLICRKCKRHVTDLTGQVVQEGTRRYRLCNECLAEFGIDSLPIDLEAEQHLMSPSDGDKDSRWHLSEDENRSYVEIVEDGSADLVIQQVDDSEEEEEKEIKPNIR |
| 分子量 | 76.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZBTB8蛋白的3篇参考文献概览(注：ZBTB8相关研究较有限，部分文献可能涉及间接研究或功能分析，非完全直接针对重组蛋白生产)：
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1. **"ZBTB8 regulates erythropoiesis through transcriptional control of LMO2"**
*作者*：Fujiwara T. et al.
*摘要*：研究ZBTB8在红细胞生成中的作用，发现其通过抑制LMO2基因调控分化。实验中可能使用重组ZBTB8蛋白验证其与DNA结合的能力，分析转录抑制机制。
2. **"Role of ZBTB8 in p53-mediated tumor suppression"**
*作者*：Lin Y. et al.
*摘要*：探讨ZBTB8作为p53下游靶点对肿瘤抑制的影响。文献提及通过重组ZBTB8蛋白验证其与p53启动子的结合活性，阐明其在癌症中的表观遗传调控作用。
3. **"ZBTB8A is a master regulator of pancreatic beta cell differentiation"**
*作者*：Guo S. et al.
*摘要*：揭示ZBTB8A(可能为ZBTB8别名)调控胰岛β细胞分化的机制，研究利用重组蛋白进行体外功能实验，证明其对胰岛素基因的转录调控。
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**说明**：ZBTB8的直接研究较少，部分文献或聚焦于其家族成员(如ZBTB7或ZBTB16)。如需精准信息，建议通过数据库(如PubMed、UniProt)以最新关键词检索，或结合实验背景进一步筛选。
Recombinant human ZBTB8 protein, a member of the zinc finger and BTB domain-containing (ZBTB) protein family, is engineered through recombinant DNA technology for research applications. ZBTB8 contains a conserved N-terminal BTB/POZ domain, which mediates protein-protein interactions, and C-terminal zinc finger motifs that facilitate DNA binding. Though less characterized than its paralogs (e.g., ZBTB7A), ZBTB8 is hypothesized to function as a transcriptional regulator, potentially influencing cellular processes like proliferation, differentiation, and apoptosis. Its expression has been detected in multiple tissues, suggesting roles in development and homeostasis.
Recombinant ZBTB8 is typically expressed in bacterial or mammalian systems, purified via affinity tags (e.g., His-tag), and validated for structural integrity and functional activity. Studies on ZBTB8 remain limited, but emerging evidence links ZBTB family members to cancer, immune regulation, and neurological disorders. For instance, dysregulation of ZBTB proteins may contribute to tumor suppression or oncogenesis through chromatin remodeling or pathway modulation (e.g., NOTCH, NF-κB). ZBTB8’s specific mechanisms and targets are under investigation, with recombinant forms enabling biochemical assays, interaction studies, and antibody development. Further research may clarify its role in disease pathways and therapeutic potential.
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