| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB12 |
| Uniprot No | Q9Y330 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459 aa |
| 活性数据 | MASGVEVLRFQLPGHEAATLRNMNQLRAEERFCDVTIVADSLKFRGHKVILAACSPFLRDQFLLNPSSELQVSLMHSARIVADLLLSCYTGALEFAVRDIVNYLTAASYLQMEHVVEKCRNALSQFIEPKIGLKEDGVSEASLVSSISATKSLLPPARTPKPAPKPPPPPPLPPPLLRPVKLEFPLDEDLELKAEEEDEDEDEDVSDICIVKVESALEVAHRLKPPGGLGGGLGIGGSVGGHLGELAQSSVPPSTVAPPQGVVKACYSLSEDAEGEGLLLIPGGRASVGATSGLVEAAAVAMAARGAGGSLGAGGSRGPLPGGFSGGNPLKNIKCTKCPEVFQGVEKLVFHMRAQHFIFMCPRCGKQFNHSSNLNRHMNVHRGVKSHSCGICGKCFTQKSTLHDHLNLHSGARPYRCSYCDVRFAHKPAIRRHLKEQHGKTTAENVLEASVAEINVLIR |
| 分子量 | 75.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人ZBTB12蛋白相关的三篇参考文献示例(注：文献信息为模拟概括，实际研究中需根据具体文献调整)：
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1. **文献名称**: *Structural insights into the ZBTB12 protein and its role in transcriptional repression*
**作者**: Kim J, et al.
**摘要**: 本研究通过X射线晶体学解析了ZBTB12蛋白的DNA结合结构域结构，揭示了其通过BTB/POZ结构域与靶基因启动子结合，抑制肿瘤相关基因表达的分子机制。
2. **文献名称**: *ZBTB12 regulates hematopoietic stem cell differentiation via epigenetic modulation*
**作者**: Müller S, et al.
**摘要**: 研究发现ZBTB12在造血干细胞分化中起关键作用，通过招募组蛋白去乙酰化酶复合物(HDACs)修饰染色质，影响细胞命运决定，为血液疾病治疗提供潜在靶点。
3. **文献名称**: *Recombinant expression of ZBTB12 in E. coli and its functional characterization in cancer cell proliferation*
**作者**: Zhang L, et al.
**摘要**: 报道了一种优化重组人ZBTB12蛋白在大肠杆菌中的可溶性表达方法，并证实其通过抑制PI3K/AKT通路显著降低多种癌细胞系的增殖能力。
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如需查找真实文献，建议在PubMed或Google Scholar检索关键词“ZBTB12 recombinant”、“ZBTB12 function”等，优先选择近五年内发表的分子生物学或肿瘤学期刊文章。
Recombinant human ZBTB12 protein is a genetically engineered form of the zinc finger and BTB domain-containing protein 12. a member of the ZBTB family of transcription regulators. ZBTB proteins are characterized by their conserved BTB (Bric-à-brac, Tramtrack, Broad-complex) domain, which mediates protein-protein interactions, and zinc finger motifs that enable sequence-specific DNA binding. ZBTB12. though less studied than other family members like ZBTB7A or ZBTB16. is implicated in transcriptional repression and may play roles in cellular processes such as differentiation, apoptosis, or oncogenesis. Its exact physiological targets and mechanisms remain under investigation, but studies suggest potential interactions with chromatin-modifying enzymes or other transcription factors to regulate gene expression networks.
The recombinant form is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) using DNA sequences encoding human ZBTB12. enabling controlled production of purified protein for research. This allows functional studies, including DNA binding assays, structural analysis (e.g., crystallography), and exploration of its role in disease contexts. Altered ZBTB12 expression has been tentatively linked to cancers and immune disorders, though evidence remains preliminary. Researchers utilize recombinant ZBTB12 to map its interactome, characterize post-translational modifications, or develop diagnostic/therapeutic strategies targeting ZBTB12-associated pathways. As with many zinc finger proteins, its modular structure also makes it a candidate for engineering synthetic transcription factors.
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