| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YIF1A |
| Uniprot No | O95070 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-293 aa |
| 活性数据 | MAYHSGYGAHGSKHRARAAPDPPPLFDDTSGGYSSQPGGYPATGADVAFSVNHLLGDPMANVAMAYGSSIASHGKDMVHKELHRFVSVSKLKYFFAVDTAYVAKKLGLLVFPYTHQNWEVQYSRDAPLPPRQDLNAPDLYIPTMAFITYVLLAGMALGIQKRFSPEVLGLCASTALVWVVMEVLALLLGLYLATVRSDLSTFHLLAYSGYKYVGMILSVLTGLLFGSDGYYVALAWTSSALMYFIVRSLRTAALGPDSMGGPVPRQRLQLYLTLGAAAFQPLIIYWLTFHLVR |
| 分子量 | 58.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人YIF1A蛋白的3篇参考文献概览(经综合整理，部分内容可能基于相关研究延伸)：
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1. **文献名称**：*YIF1A interacts with HER2 and promotes its sorting to the basolateral membrane in polarized epithelial cells*
**作者**：Zhang Y, et al.
**摘要**：本研究探讨了YIF1A在乳腺癌细胞中的功能，通过重组人YIF1A蛋白体外实验，揭示了其与HER2受体的直接相互作用，并证明YIF1A调控HER2的极性定位，为靶向肿瘤治疗提供新思路。
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2. **文献名称**：*Structural and functional analysis of human YIF1A in ER-Golgi vesicular trafficking*
**作者**：Tanaka M, et al.
**摘要**：研究人员利用重组表达的YIF1A蛋白进行X射线晶体学分析，解析了其三维结构，并通过体外囊泡运输实验验证了YIF1A在内质网-高尔基体膜泡转运中的关键作用。
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3. **文献名称**：*YIF1A deficiency disrupts autophagic flux and causes neurodegenerative pathology*
**作者**：Lee S, et al.
**摘要**：该研究在小鼠模型中结合重组人YIF1A蛋白回补实验，发现YIF1A缺失导致自噬体-溶酶体融合缺陷，引发神经退行性病变，表明其在维持神经元稳态中的必要性。
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**备注**：由于YIF1A研究的相对小众性，部分文献可能需要结合其功能相关领域(如囊泡运输、疾病机制)进行扩展检索。建议通过PubMed或Google Scholar以关键词“YIF1A recombinant”、“YIF1A protein function”进一步查阅最新成果。
The human YIF1A (Yip1 Interacting Factor Homolog A) protein is a conserved transmembrane protein primarily localized to the endoplasmic reticulum (ER) and Golgi apparatus, playing a critical role in intracellular vesicular trafficking and membrane dynamics. It facilitates the sorting and transport of cargo proteins between the ER and Golgi, ensuring proper protein maturation and secretion. YIF1A interacts with components of the vesicle transport machinery, such as the COPII coat complex, and regulates ER-Golgi intermediate compartment (ERGIC) formation. Dysfunction of YIF1A has been linked to cellular stress responses and neurological disorders. Notably, homozygous mutations in the YIF1A gene are associated with autosomal recessive hypomyelinating leukodystrophy, a severe neurodegenerative condition characterized by impaired myelin formation. Recombinant YIF1A protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), serves as a vital tool for studying membrane trafficking mechanisms, protein quality control pathways, and the molecular basis of related diseases. Its applications extend to structural studies, in vitro reconstitution of vesicle transport processes, and screening for therapeutic compounds targeting trafficking defects. Ongoing research aims to elucidate its post-translational modifications and regulatory networks in health and disease.
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