| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YBX2 |
| Uniprot No | Q9Y2T7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364 aa |
| 活性数据 | MSEVEAAAVATAVPAATVPATAAGVVAVVVPVPAGEPQKGGGAGGGGGAASGPAAGTPSAPGSRTPGNPATAVSGTPAPPARSQADKPVLAIQVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKRNNPRKFLRSVGDGETVEFDVVEGEKGAEATNVTGPGGVPVKGSRYAPNRRKSRRFIPRPPSVAPPPMVAEIPSAGTGPGSKGERAEDSGQRPRRWCPPPFFYRRRFVRGPRPPNQQQPIEGTDRVEPKETAPLEGHQQQGDERVPPPRFRPRYRRPFRPRPRQQPTTEGGDGETKPSQGPADGSRPEPQRPRNRPYFQRRRQQAPGPQQAPGPRQPAAPETSAPVNSGDPTTTILE |
| 分子量 | 65 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：*YBX2 is required for the maintenance of spermatogial stem cells in mice*
**作者**：Yang, Q., et al.
**摘要**：研究通过基因敲除模型发现YBX2对小鼠精原干细胞的维持至关重要，重组YBX2蛋白实验证实其通过调控特定mRNA稳定性影响生殖细胞发育。
2. **文献名称**：*MSY2 (YBX2) is essential for postnatal development of male germ cells*
**作者**：Gu, W., et al.
**摘要**：报道重组人YBX2蛋白在体外结合生殖细胞mRNA的机制，证明其在精子形成阶段通过选择性翻译调控维持男性生殖细胞存活和分化。
3. **文献名称**：*Structural basis of YBX2 recognition of pre-methylated mRNA*
**作者**：Zhang, L., et al.
**摘要**：通过X射线晶体学解析重组YBX2蛋白与甲基化RNA的复合物结构，揭示其特异性识别并结合mRNA 5’端修饰的分子机制。
4. **文献名称**：*YBX2 promotes ovarian cancer progression via enhancing mitochondrial metabolism*
**作者**：Chen, R., et al.
**摘要**：发现重组YBX2蛋白通过激活线粒体相关基因表达促进卵巢癌细胞代谢重编程，提示其作为癌症治疗靶点的潜力。
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*注：以上文献为示例性内容，实际引用时需核查真实数据库(如PubMed)中的具体信息。YBX2亦被称为MSY2.检索时可尝试别名扩展。*
Recombinant human YBX2 protein, also known as Y-box binding protein 2. is a member of the Y-box protein family characterized by its evolutionarily conserved cold shock domain (CSD). YBX2 is encoded by the YBX2 gene located on chromosome 17 in humans. Structurally, it contains a central CSD that facilitates binding to RNA or single-stranded DNA, flanked by variable N- and C-terminal regions involved in protein-protein interactions. YBX2 is predominantly expressed in germline cells and plays critical roles in gametogenesis. During spermatogenesis, it regulates mRNA stability, translational repression, and storage in male germ cells. In oocytes, it is essential for maintaining mRNA dormancy and activating translation during maturation. Studies also link YBX2 to chromatin remodeling and epigenetic regulation during early embryogenesis. Dysregulation of YBX2 has been implicated in infertility and certain cancers, where it may promote tumor progression by modulating oncogenic transcripts. Recombinant YBX2 proteins are produced using heterologous expression systems (e.g., E. coli, mammalian cells) to study its molecular interactions, structural dynamics, and functional mechanisms in vitro. This tool enables researchers to dissect its roles in post-transcriptional gene regulation, germ cell development, and potential therapeutic targeting.
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