| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XAB2 |
| Uniprot No | Q9HCS7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-855 aa |
| 活性数据 | MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERALKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFLMDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESAEEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNVDAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYAQFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEWHKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEKATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRVYKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKAYERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYAQLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLSDEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTIKEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLAAEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLEQQSVPAAVFGSLKED |
| 分子量 | 119.79 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人XAB2蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *XAB2. a novel tetratricopeptide repeat protein involved in transcription and DNA repair*
**作者**: Kuraoka I, et al.
**摘要**: 该研究报道了重组人XAB2蛋白在转录耦合修复(TCR)中的作用。作者通过体外实验证明，XAB2与RNA聚合酶II及DNA损伤修复因子(如CSA/CSB)相互作用，并通过其TPR结构域介导修复复合物的组装。重组XAB2蛋白的缺失导致紫外线诱导的DNA损伤修复效率显著降低。
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2. **文献名称**: *Mutations in XAB2 cause a severe neurodevelopmental syndrome with impaired DNA repair*
**作者**: Tory K, et al.
**摘要**: 本研究利用重组XAB2蛋白功能分析，揭示了XAB2基因突变与人类神经发育障碍的相关性。实验显示，重组突变型XAB2蛋白丧失与转录延伸因子相互作用的能力，导致细胞对DNA损伤敏感，并干扰神经细胞增殖和分化。
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3. **文献名称**: *Structural and functional insights into the role of XAB2 in coordinating R-loop resolution and transcription*
**作者**: Sollier J, et al.
**摘要**: 通过重组人XAB2蛋白的生化分析，本文揭示了XAB2通过调控R环(转录过程中形成的RNA-DNA杂合体)的稳定性来维持基因组稳定性。研究发现，XAB2通过结合单链DNA和RNA，协调转录与同源重组修复之间的平衡。
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**备注**：以上文献为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索。建议以“XAB2 recombinant protein”或“XAB2 DNA repair”为关键词查找近期研究。
**Background of Recombinant Human XAB2 Protein**
XAB2 (XPA-binding protein 2) is a multifunctional protein involved in transcription-coupled DNA repair, RNA splicing, and transcriptional regulation. It was initially identified through its interaction with XPA, a critical component of the nucleotide excision repair (NER) pathway responsible for removing bulky DNA lesions. XAB2 plays a central role in transcription-coupled repair (TCR), a subpathway of NER that preferentially repairs DNA damage in transcriptionally active regions. By associating with RNA polymerase II and spliceosome components, XAB2 facilitates crosstalk between transcription, splicing, and DNA repair machinery.
Structurally, XAB2 contains multiple tetratricopeptide repeat (TPR) domains, enabling interactions with diverse partners, including the mRNA splicing factor PRP19 and the transcription elongation factor ELL2. Studies suggest that XAB2 coordinates the recruitment of repair factors to sites of transcription-blocking lesions, ensuring genomic stability. Dysregulation of XAB2 has been implicated in cellular sensitivity to DNA damage, developmental defects, and diseases such as cancer.
Recombinant human XAB2 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains its functional domains and is widely used to study its biochemical interactions, repair mechanisms, and structural properties. Research on XAB2 continues to elucidate its dual roles in maintaining genomic integrity and regulating gene expression, highlighting its potential as a therapeutic target in DNA repair-deficient disorders.
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