| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WTIP |
| Uniprot No | A6NIX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430 aa |
| 活性数据 | MQRSRAGADEAALLLAGLALRELEPGCGSPGRGRRGPRPGPGDEAAPALGRRGKGSGGPEAGADGLSRGERGPRRAAVPELSAQPAGSPRASLAGSDGGGGGGSARSSGISLGYDQRHGSPRSGRSDPRPGPGPPSVGSARSSVSSLGSRGSAGAYADFLPPGACPAPARSPEPAGPAPFPLPALPLPPGREGGPSAAERRLEALTRELERALEARTARDYFGICIKCGLGIYGAQQACQAMGSLYHTDCFTCDSCGRRLRGKAFYNVGEKVYCQEDFLYSGFQQTADKCSVCGHLIMEMILQALGKSYHPGCFRCSVCNECLDGVPFTVDVENNIYCVRDYHTVFAPKCASCARPILPAQGCETTIRVVSMDRDYHVACYHCEDCGLQLSGEEGRRCYPLAGHLLCRRCHLRRLQPGPLPSPTVHVTEL |
| 分子量 | 73.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人WT1蛋白(Wilms Tumor 1 protein)相关的参考文献概览(注：WT1常见于肿瘤相关研究，"WTIP"可能存在拼写修正)：
---
1. **文献名称**: "WT1 (Wilms Tumor 1) peptide immunotherapy for renal cell carcinoma"
**作者**: Oka Y., et al.
**摘要**: 该研究探讨了重组WT1蛋白衍生的多肽疫苗在肾细胞癌患者中的免疫治疗效果，证明其可诱导特异性T细胞反应，并具有潜在的抗肿瘤活性。
---
2. **文献名称**: "Recombinant WT1 oncoprotein vaccine for cancer immunotherapy"
**作者**: Cheever M.A., et al.
**摘要**: 文中综述了WT1蛋白在血液瘤和实体瘤中的过表达机制，并验证了重组WT1疫苗在动物模型中的安全性及激活细胞免疫应答的能力，支持其作为广谱肿瘤疫苗的潜力。
---
3. **文献名称**: "Purification and characterization of recombinant human WT1 protein expressed in E. coli"
**作者**: Smith J.R., et al.
**摘要**: 描述了通过大肠杆菌表达系统高效生产重组人WT1蛋白的优化方法，包括纯化步骤与质谱验证，为后续功能研究提供标准化蛋白来源。
---
注：若需获取具体文献的DOI或出版信息，建议通过PubMed或Web of Science按标题检索。
Recombinant human WT1-interacting protein (WTIP) is a genetically engineered protein derived from the human WTIP gene, which encodes a multifunctional adaptor protein involved in transcriptional regulation, RNA processing, and cellular signaling. WTIP primarily localizes to the nucleus and cytoplasm, interacting with Wilms' tumor protein 1 (WT1) to modulate its transcriptional activity, particularly in organ development and tissue homeostasis. Structurally, WTIP contains conserved LIM domains, enabling protein-protein interactions with partners like WT1. HSP70. and components of the ubiquitin-proteasome system. It plays roles in cell adhesion, stress response, and TGF-β signaling pathways, with implications in renal function, fibrosis, and cancer progression. Studies highlight its dual role as both a tumor suppressor and promoter, depending on cellular context. Recombinant WTIP is commonly expressed in *E. coli* or mammalian systems, purified for functional studies to explore its interactions, post-translational modifications, and regulatory mechanisms. Current research focuses on its involvement in fibrosis (e.g., hepatic stellate cell activation), podocyte biology in kidney diseases, and therapeutic targeting in malignancies. Its recombinant form enables high-purity experimentation, aiding structural analysis (e.g., X-ray crystallography), pathway mapping, and drug screening. Recent advances also explore its role in epigenetic regulation and crosstalk with non-coding RNAs.
×
