| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDK2 |
| Uniprot No | Q15119 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-407aa |
| 氨基酸序列 | MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDK2重组蛋白的3篇参考文献，涵盖表达纯化、功能研究和应用方向：
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1. **文献名称**：*Expression, purification, and biochemical characterization of recombinant human pyruvate dehydrogenase kinase 2 (PDK2)*
**作者**：Bowker-Kinley MM, Davis WI, Wu P, et al.
**摘要**：该研究报道了人源PDK2在大肠杆菌中的重组表达及纯化方法，分析了其激酶活性及对丙酮酸脱氢酶复合体(PDC)的磷酸化调控作用，为PDK2的酶学特性研究提供了基础工具。
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2. **文献名称**：*Structural insights into the inhibitory mechanism of novel PDK2 inhibitors for diabetes therapy*
**作者**：Kato M, Li J, Chuang DT, et al.
**摘要**：通过昆虫细胞系统表达并纯化重组PDK2蛋白，结合X射线晶体学解析其三维结构，筛选出小分子抑制剂，阐明其结合模式，为2型糖尿病药物开发提供结构基础。
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3. **文献名称**：*Recombinant PDK2 as a potential therapeutic target in cancer metabolism*
**作者**：Kim JW, Dang CV, Sutendra G, et al.
**摘要**：利用重组PDK2蛋白探究其在肿瘤细胞代谢重编程中的作用，发现抑制PDK2可逆转Warburg效应，增强线粒体氧化磷酸化，为癌症靶向治疗提供新策略。
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**备注**：若需补充更多文献或特定研究方向(如PDK2重组蛋白的动力学分析)，可进一步调整检索关键词(如“PDK2 recombinant kinetic”或“PDK2 protein production”)。
**Background of PDK2 Recombinant Protein**
Pyruvate dehydrogenase kinase 2 (PDK2) is a mitochondrial enzyme that plays a critical role in regulating cellular energy metabolism. It belongs to the PDK family (PDK1-4), which phosphorylates and inhibits the pyruvate dehydrogenase complex (PDC), a key enzyme complex responsible for converting pyruvate into acetyl-CoA. This reaction links glycolysis to the tricarboxylic acid (TCA) cycle, and its regulation by PDK2 ensures metabolic flexibility under varying nutrient conditions.
PDK2 is particularly significant in balancing glucose oxidation and lipid metabolism. During high glucose availability, PDK2 activity increases, suppressing PDC to limit acetyl-CoA entry into the TCA cycle. This shifts cells toward glycolysis and lactate production, a phenomenon observed in cancers (Warburg effect) and metabolic disorders. Conversely, PDK2 downregulation promotes glucose oxidation, enhancing mitochondrial respiration.
Recombinant PDK2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce functional, purified PDK2 for research and therapeutic applications. Its production enables studies on enzyme kinetics, structural biology, and interactions with inhibitors or activators. PDK2 is a target for metabolic diseases, such as type 2 diabetes, as its inhibition may improve glucose utilization. In cancer, PDK2 overexpression is linked to tumor progression, making it a potential therapeutic target.
Research on PDK2 recombinant protein also aids in understanding its role in ischemia-reperfusion injury, neurodegenerative diseases, and aging. Structural insights from recombinant PDK2 have guided drug design, aiming to modulate its activity with high specificity. Overall, PDK2 recombinant protein serves as a vital tool for dissecting metabolic regulation and developing precision therapies.
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