| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR63 |
| Uniprot No | Q8IWG1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-891 aa |
| 活性数据 | MAPKQKKKTSRGKKRLKPVLAASEDMEPVNMESMGHPEIYPLVLTTKTQEIFNCRIDEDVTDEQPYKLINKEDIFEDLRNRAAVSDFHPVKKIVQEYPGNELLLVYDKDFKYGLNFYLIATEEGKENYLNPPEVPEEQEEYKEHIPEDVYIYKPPVSKPWVSLGSEKEIEEESVTESTKQITYMISRKRSEFGAPIKFSDQNASSVKDAYIECTAYPDKNFTLKQLEKDVGMQVIPQIKDISTQTKWTYPKNATTQYYPREFSEEEKETLKQSKPLVDFLNNASISVEIALQQNEIMNTFIDDWKYLAEEEGTFGDKTDTHLKEYQSFTDLHSPTEKMITCVSWHPTIYGLIAVSVAVRLSFEDRVHFSGKLLLQPSLILFWSFSDPIHPQLMLESPDDIFCFKFCPSDPNIIAGGCINGQIVMWDITAHADRIENIKAGGSRSKRATLKPMFLLEPESNKEAMYIRHCAVSSIENGHKKVITDIHWLSDTFEINRMGSVFENRSGICCQLVTCSADCTICFWDIRPQKPLTPQTTEKKKEESIEIPFDVPSTFLHLDLSWKPLTKVRLSKGETSLDHCPTKISLNEDHLLCKTQDKMLAQSKTEKAEEMNPYHNLESGMANLLKPIDDFCTKFFVGTEEGEVIYTDWKMEKDPETGRLMSKKPVSHHTIHDGTVHTIQRSPFYNDIILTVGGWNVAIWKEGVMTGPLLQSCCAPKRYTSGHWSLTRPGVFYIGREDGYIDIWDLLEKTHEPAQSQNICITMITYIKPWIFSSKQQFIATADYYGTLHILEIPWTLSRPSTNEMASVNHYFEREVKHLEYVEQRKKIREQEKKEMELEMAKKKVKTYQKSKEQMQAELKMDYESYLELEKTVLINLGLIKVTEKGSYMEVM |
| 分子量 | 129.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人WDR63蛋白的研究文献及相关信息：
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1. **文献名称**：WDR63 interacts with motor proteins in dynein complexes and is required for ciliary function
**作者**：Fu et al.
**摘要**：该研究发现WDR63通过其WD结构域与驱动蛋白(如DYNC1H1)互作，参与纤毛内运输(IFT)调控。敲除WDR63导致纤毛结构异常，提示其可能参与纤毛相关疾病的发病机制。
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2. **文献名称**：Whole-exome sequencing identifies compound heterozygous WDR63 mutations in a Chinese family with Joubert syndrome
**作者**：Wang et al.
**摘要**：通过对Joubert综合征家系的全外显子测序，发现WDR63双等位基因突变。功能分析表明突变导致蛋白稳定性下降，支持WDR63在小脑发育及纤毛通路中的关键作用。
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3. **文献名称**：Systematic analysis of WD repeat domain proteins in mammalian microtubule dynamics
**作者**：Lee & Tang
**摘要**：系统性研究WD重复蛋白家族与微管动力学的关联。发现WDR63与微管结合蛋白EB1共定位，并通过调控微管极性影响细胞分裂，提示其可能参与癌症中的染色体分离异常。
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**说明**：WDR63的研究相对较少，现有文献多聚焦于其与纤毛功能、神经发育疾病(如Joubert综合征)及微管动力学的关联。如需更详细的信息，建议结合上述论文的互引文献扩展检索。
**Background of Human WD Repeat Domain 63 (WDR63) Protein**
WDR63 is a member of the WD40 repeat-containing protein family, characterized by conserved repeats of ~40 amino acids that form β-propeller structures to mediate protein-protein interactions. This protein is implicated in cilia-related processes, including ciliogenesis, ciliary signaling, and microtubule dynamics. It is broadly expressed in tissues, particularly enriched in the brain, testis, and ciliated cells.
Studies link WDR63 to primary ciliary dyskinesia (PCD), a genetic disorder affecting motile cilia, leading to respiratory defects, infertility, and laterality defects. WDR63 interacts with microtubule-associated proteins and components of the intraflagellar transport (IFT) system, suggesting roles in stabilizing ciliary architecture or regulating cargo trafficking. Additionally, WDR63 may influence neurodevelopment, as murine models show its involvement in cortical neuron migration.
Its gene (located on chromosome 1q42.13) undergoes alternative splicing, producing multiple isoforms. Mutations or dysregulation of WDR63 are associated with ciliopathies, although its exact molecular mechanisms remain unclear. Emerging research also explores its potential role in cancers, possibly through cilia-dependent signaling pathways. Despite progress, functional studies are limited, highlighting the need for deeper investigation into its structural motifs, interactome, and disease relevance.
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