| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VDAC2 |
| Uniprot No | P45880 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-283 aa |
| 活性数据 | MCIPPSYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGVEFSTSGSSNTDTGKVTGTLETKYKWCEYGLTFTEKWNTDNTLGTEIAIEDQICQGLKLTFDTTFSPNTGKKSGKIKSSYKRECINLGCDVDFDFAGPAIHGSAVFGYEGWLAGYQMTFDSAKSKLTRNNFAVGYRTGDFQLHTNVNDGTEFGGSIYQKVCEDLDTSVNLAWTSGTNCTRFGIAAKYQLDPTASISAKVNNSSLIGVGYTQTLRPGVKLTLSALVDGKSINAGGHKVGLALELEA |
| 分子量 | 56.65 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VDAC2蛋白的3篇示例参考文献(注：文献信息为示例性概括，可能不指向实际已发表论文)：
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1. **文献名称**：*"Functional characterization of recombinant human VDAC2 in mitochondrial apoptosis"*
**作者**：Cheng, E.H., et al.
**摘要**：研究利用重组表达的VDAC2蛋白，揭示其通过特异性结合促凋亡蛋白Bak调控线粒体外膜通透性，抑制细胞凋亡的分子机制，为癌症治疗提供潜在靶点。
2. **文献名称**：*"Expression and electrophysiological analysis of recombinant VDAC2 channels"*
**作者**：Maes, M., et al.
**摘要**：报道在大肠杆菌中高效表达重组人VDAC2蛋白，纯化后通过脂质双层电生理实验证实其形成阴离子选择性通道，并表现出与VDAC1不同的电压门控特性。
3. **文献名称**：*"VDAC2-mediated calcium transport in reconstituted lipid membranes"*
**作者**：De Stefani, D., et al.
**摘要**：利用重组VDAC2重构脂质体模型，发现其参与调控线粒体钙离子吸收，并揭示钙信号异常与神经退行性疾病的相关性。
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以上内容基于VDAC2已知功能领域的合理推测，实际引用时建议通过学术数据库(如PubMed)核实具体文献。
Voltage-Dependent Anion Channel 2 (VDAC2) is a mitochondrial outer membrane protein belonging to the VDAC family, which includes three isoforms (VDAC1. VDAC2. and VDAC3) in humans. As a key component of the mitochondrial permeability transition pore, VDAC2 facilitates the transport of metabolites, ions, and nucleotides across the mitochondrial membrane, playing a critical role in cellular energy metabolism and apoptosis regulation. Unlike VDAC1 (the most abundant isoform), VDAC2 exhibits distinct tissue-specific expression patterns and unique functional roles. It is particularly enriched in tissues with high metabolic demands, such as the heart, brain, and testes.
Structurally, VDAC2 adopts a β-barrel conformation with a conserved N-terminal α-helix that modulates channel gating and substrate selectivity. Recombinant human VDAC2 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) followed by purification via affinity chromatography. Its recombinant form enables detailed studies on mitochondrial physiology, including interactions with pro-apoptotic Bcl-2 family proteins like BAK, which are crucial for apoptosis initiation. VDAC2 also shows potential involvement in pathologies such as cancer, neurodegenerative disorders, and cardiovascular diseases, making it a therapeutic target. Current research focuses on deciphering its isoform-specific functions, post-translational modifications, and role in mitochondrial quality control mechanisms.
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