| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VAX2 |
| Uniprot No | Q9UIW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-290 aa |
| 活性数据 | MGDGGAERDR GPARRAESGG GGGRCGDRSG AGDLRADGGG HSPTEVAGTS ASSPAGSRES GADSDGQPGP GEADHCRRIL VRDAKGTIRE IVLPKGLDLD RPKRTRTSFT AEQLYRLEME FQRCQYVVGR ERTELARQLN LSETQVKVWF QNRRTKQKKD QSRDLEKRAS SSASEAFATS NILRLLEQGR LLSVPRAPSL LALTPSLPGL PASHRGTSLG DPRNSSPRLN PLSSASASPP LPPPLPAVCF SSAPLLDLPA GYELGSSAFE PYSWLERKVG SASSCKKANT |
| 分子量 | 30.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人VAX2蛋白相关的虚构参考文献范例(实际文献需通过学术数据库查询)：
1. **文献名称**：*"Functional Characterization of Recombinant Human VAX2 Protein in Retinal Development"*
**作者**：Smith, J. et al. (2015)
**摘要**：本研究通过在大肠杆菌系统中成功表达并纯化重组人VAX2蛋白，发现其与特定DNA序列结合的能力，并证实其在调控视网膜神经细胞分化中的关键作用。实验表明，VAX2的缺失会导致小鼠胚胎眼部发育异常。
2. **文献名称**：*"Structural Analysis of VAX2 Homeodomain Using X-ray Crystallography"*
**作者**：Chen, L. et al. (2018)
**摘要**：通过重组表达人VAX2蛋白的DNA结合结构域，结合X射线晶体学揭示了其三维结构，并发现该结构域与靶基因启动子的相互作用机制，为解释VAX2突变相关先天性眼病提供了分子基础。
3. **文献名称**：*"Recombinant VAX2 Protein Modulates Wnt Signaling in vitro"*
**作者**：Braasch, K. et al. (2020)
**摘要**：利用哺乳动物细胞表达系统制备重组人VAX2蛋白，发现其通过调控Wnt/β-catenin通路抑制视网膜色素上皮细胞增殖，提示VAX2在眼组织稳态维持中的潜在应用价值。
4. **文献名称**：*"Optimization of Recombinant VAX2 Production in HEK293 Cells for Therapeutic Screening"*
**作者**：Gómez-Martín, R. et al. (2019)
**摘要**：开发了一种高效的重组VAX2蛋白哺乳动物表达系统，优化纯化工艺后获得高活性蛋白，并用于筛选小分子药物以治疗VAX2缺陷相关的视力障碍疾病。
(注：以上内容为模拟数据，实际研究需参考PubMed、Web of Science等平台。)
Recombinant human VAX2 protein is a genetically engineered form of the ventral anterior homeobox 2 (VAX2) protein, a transcription factor belonging to the homeobox gene family. VAX2 plays a critical role in embryonic development, particularly in patterning the ventral retina and establishing visual pathway connectivity. It regulates axon guidance and neural circuit formation in the visual system by controlling the expression of guidance molecules like ephrins. Mutations or dysregulation of VAX2 have been linked to ocular disorders, including microphthalmia and abnormal optic nerve development.
The recombinant protein is typically produced in bacterial or mammalian expression systems, enabling studies of its structure, DNA-binding properties, and interaction partners. Its applications span basic research—elucidating mechanisms of retinal development, neuronal connectivity, and gene regulatory networks—and translational studies, such as screening for therapeutic compounds targeting VAX2-related disorders. Purified recombinant VAX2 retains functional activity, making it valuable for in vitro assays, protein interaction analyses, and structural biology. Recent work also explores its potential in regenerative medicine, particularly in guiding stem cell differentiation toward retinal lineages. Despite progress, further research is needed to fully decode its downstream targets and tissue-specific roles.
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