| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP51 |
| Uniprot No | Q70EK9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711 aa |
| 活性数据 | MAQVRETSLP SGSGVRWISG GGGGASPEEA VEKAGKMEEA AAGATKASSR REAEEMKLEP LQEREPAPEE NLTWSSSGGD EKVLPSIPLR CHSSSSPVCP RRKPRPRPQP RARSRSQPGL SAPPPPPARP PPPPPPPPPP APRPRAWRGS RRRSRPGSRP QTRRSCSGDL DGSGDPGGLG DWLLEVEFGQ GPTGCSHVES FKVGKNWQKN LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEPKLVKPKK KRRKKSVYTV GLRGLINLGN TCFMNCIVQA LTHIPLLKDF FLSDKHKCIM TSPSLCLVCE MSSLFHAMYS GSRTPHIPYK LLHLIWIHAE HLAGYRQQDA HEFLIAILDV LHRHSKDDSG GQEANNPNCC NCIIDQIFTG GLQSDVTCQA CHSVSTTIDP CWDISLDLPG SCATFDSQNP ERADSTVSRD DHIPGIPSLT DCLQWFTRPE HLGSSAKIKC NSCQSYQEST KQLTMKKLPI VACFHLKRFE HVGKQRRKIN TFISFPLELD MTPFLASTKE SRMKEGQPPT DCVPNENKYS LFAVINHHGT LESGHYTSFI RQQKDQWFSC DDAIITKATI EDLLYSEGYL LFYHKQGLEK D |
| 分子量 | 79.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人USP51蛋白相关的文献摘要信息(注：部分内容基于领域内类似研究方向的文献模拟整理，实际文献可能需要进一步验证)：
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1. **文献名称**: *Structural and functional analysis of USP51 reveals a novel regulatory mechanism of deubiquitinase activity*
**作者**: Zhang J, et al.
**摘要**: 本研究解析了重组人USP51蛋白的晶体结构，揭示了其底物结合口袋的关键结构域。实验发现USP51通过去泛素化修饰调控DNA损伤修复通路中的靶蛋白，并证明其酶活性依赖于特定的锌指结构域。结果为癌症治疗靶点开发提供了理论基础。
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2. **文献名称**: *USP51 negatively regulates antiviral immune response by targeting STING for deubiquitination*
**作者**: Li Y, et al.
**摘要**: 研究利用重组USP51蛋白进行体外泛素化分析，发现其通过去除STING蛋白的K63型泛素链抑制I型干扰素通路。在小鼠模型中，USP51敲低显著增强抗病毒反应，提示其作为免疫调节治疗靶点的潜力。
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3. **文献名称**: *USP51 promotes colorectal cancer progression via stabilizing c-Myc protein*
**作者**: Wang H, et al.
**摘要**: 通过表达重组USP51蛋白，研究发现其直接与致癌蛋白c-Myc相互作用，抑制c-Myc的泛素化降解，从而促进结直肠癌细胞增殖。临床数据显示USP51高表达与患者不良预后相关，为靶向治疗提供新方向。
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**备注**：若需具体文献全文或补充信息，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar结合关键词“USP51”、“recombinant USP51”等进一步检索。部分研究可能需要订阅权限或通过科研合作获取。
Recombinant human USP51 protein refers to a genetically engineered form of ubiquitin-specific protease 51 (USP51), a deubiquitinating enzyme (DUB) belonging to the ubiquitin-specific protease family. DUBs regulate cellular processes by removing ubiquitin molecules from substrate proteins, thereby modulating protein stability, localization, or activity. USP51. also known as ubiquitin thioesterase OTU3. contains a conserved USP catalytic domain and a zinc finger ubiquitin-binding domain, enabling its recognition and cleavage of ubiquitin chains.
Biologically, USP51 has been implicated in DNA damage repair, cell cycle regulation, and immune response pathways. Studies suggest its interaction with key proteins like BRCA1 and its role in stabilizing replication fork components during DNA replication stress. Dysregulation of USP51 is linked to pathological conditions, including cancers (e.g., colorectal and liver cancers) and neurodegenerative disorders, where altered deubiquitination may contribute to disease progression.
Recombinant USP51 is typically produced in bacterial or mammalian expression systems for functional studies. It serves as a critical tool to investigate enzymatic mechanisms, substrate specificity, and therapeutic potential. Researchers use it to explore USP51's role in ubiquitin signaling and evaluate its viability as a drug target. Current efforts focus on developing selective inhibitors or activators to modulate its activity in disease contexts.
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