| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP4 |
| Uniprot No | Q13107 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-963 aa |
| 活性数据 | MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN |
| 分子量 | 135 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人USP4蛋白的参考文献示例(内容为示例性概括，非真实文献)：
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1. **文献名称**：*"Expression and enzymatic characterization of recombinant human USP4: Insights into substrate specificity"*
**作者**：Zhang Y. et al.
**摘要**：该研究通过在大肠杆菌系统中成功表达并纯化重组人USP4蛋白，分析了其去泛素化酶活性，揭示了USP4对K48和K63型泛素链的特异性切割能力，并探讨了其催化结构域的关键氨基酸残基。
2. **文献名称**：*"USP4 negatively regulates TGF-β signaling by deubiquitinating Smad7"*
**作者**：Xu L. et al.
**摘要**：利用重组人USP4蛋白进行体外实验，证明其通过去泛素化Smad7蛋白调控TGF-β信号通路，抑制肿瘤细胞的上皮-间质转化(EMT)，揭示了USP4在癌症转移中的潜在抑制作用。
3. **文献名称**：*"Structural basis of ubiquitin recognition and cleavage by USP4"*
**作者**：Chen J. et al.
**摘要**：通过X射线晶体学解析重组人USP4蛋白与泛素复合物的三维结构，阐明了USP4的催化机制及底物结合模式，为设计靶向USP4的小分子抑制剂提供了结构基础。
4. **文献名称**：*"USP4 promotes DNA damage repair through stabilizing HDAC2 in glioma"*
**作者**：Wang H. et al.
**摘要**：研究利用重组USP4蛋白证明其通过去泛素化并稳定HDAC2.增强胶质瘤细胞对DNA损伤的修复能力，提示USP4可能作为治疗放疗抵抗性癌症的靶点。
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**注**：以上文献信息为示例性呈现，实际引用时需查询具体数据库(如PubMed、Web of Science)获取真实文献。
Recombinant human USP4 (Ubiquitin-Specific Protease 4) is a deubiquitinating enzyme (DUB) belonging to the cysteine protease family. It plays a critical role in regulating protein degradation by selectively removing ubiquitin molecules from substrate proteins, thereby modulating their stability, localization, and activity. USP4 is involved in diverse cellular processes, including DNA repair, immune response regulation, and signal transduction pathways such as NF-κB and TGF-β. Structurally, USP4 contains characteristic domains like the N-terminal ubiquitin-like (UBL) domain and a catalytic domain responsible for its enzymatic function.
Research links USP4 to various diseases. It acts as a tumor suppressor in some cancers by stabilizing tumor-inhibiting proteins but may promote oncogenesis in others by enhancing pro-survival signals. It also influences viral infection outcomes and neurodegeneration. Recombinant USP4 protein, typically produced in bacterial or eukaryotic expression systems (e.g., E. coli or HEK293 cells), enables mechanistic studies of its interactions, enzymatic kinetics, and structure-function relationships. This recombinant tool is vital for screening DUB inhibitors, exploring therapeutic strategies for cancer, autoimmune disorders, and viral infections. Its production often includes tags (e.g., His-tag) for purification and detection, ensuring high purity and activity for experimental reproducibility.
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