| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP48 |
| Uniprot No | Q86UV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1035 aa |
| 活性数据 | MAPRLQLEKAAWRWAETVRPEEVSQEHIETAYRIWLEPCIRGVCRRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDDPNCERRKKNSFVGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAYVTVCNQCGRESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSKQNATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNTYIGFSEILDMEPYVEHKGGSYVYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKMEGKKLQLGIEEDLAEPSKSQTRKPKCGKGTHCSRNAYMLVYRLQTQEKPNTTVQVPAFLQELVDRDNSKFEEWCIEMAEMRKQSVDKGKAKHEEVKELYQRLPAGAEPYEFVSLEWLQKWLDESTPTKPIDNHACLCSHDKLHPDKISIMKRISEYAADIFYSRYGGGPRLTVKALCKECVVERCRILRLKNQLNEDYKTVNNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDEQDGDAEQSNGKMNGSTLNKDESKEERKEEEELNFNEDILCPHGELCISENERRLVSKEAWSKLQQYFPKAPEFPSYKECCSQCKILEREGEENEALHKMIANEQKTSLPNLFQDKNRPCLSNWPEDTDVLYIVSQFFVEEWRKFVRKPTRCSPVSSVGNSALLCPHGGLMFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAYQKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSIDGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGFKGTGLLGH |
| 分子量 | 145.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇基于常见研究方向的示例文献(内容为示例，建议通过学术数据库获取真实文献)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of Human USP48 in Yeast System"
**作者**: Liu, Y. et al.
**摘要**: 本研究利用酵母表达系统成功表达了重组人USP48蛋白，证实其去泛素化酶活性，并通过质谱分析鉴定了其底物特异性，为大规模生产及酶学研究提供了基础。
2. **文献名称**: "Structural Insights into USP48 Substrate Recognition via Cryo-EM Analysis"
**作者**: Kim, S. & Patel, R.
**摘要**: 通过冷冻电镜解析了重组人USP48与泛素链复合物的结构，揭示了其底物结合域的关键氨基酸残基，阐明了其去泛素化作用的分子机制。
3. **文献名称**: "USP48 Regulates DNA Repair Pathways via Recombinant Protein Interaction Screening"
**作者**: Gonzalez, L. et al.
**摘要**: 利用重组USP48蛋白进行体外互作实验，发现其与多种DNA修复蛋白(如BRCA1)的相互作用，提示其在基因组稳定性中的潜在作用。
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**建议**：可通过PubMed、Web of Science等平台，以关键词“recombinant human USP48”、“USP48 expression”、“USP48 function”检索近5年文献，获取最新研究进展。
**Background of Recombinant Human USP48 Protein**
USP48 (Ubiquitin-Specific Protease 48) is a deubiquitinating enzyme belonging to the ubiquitin-specific protease family, which plays a critical role in regulating protein stability and cellular processes by removing ubiquitin chains from target substrates. This enzyme contains a conserved catalytic domain responsible for its hydrolase activity, enabling it to reverse ubiquitination-mediated protein degradation or signaling events. USP48 is involved in diverse pathways, including transcriptional regulation, DNA repair, and immune responses, often interacting with histone-modifying complexes or stress-response machinery.
Research highlights its association with diseases such as cancer, where USP48 overexpression is linked to chemoresistance or tumor progression in certain contexts. It also influences inflammatory and neurological disorders by modulating NF-κB signaling or epigenetic regulators. Recombinant human USP48 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and is vital for *in vitro* studies to dissect its molecular mechanisms, substrate specificity, and regulatory networks. Its applications span drug screening, structural analysis, and identifying inhibitors for therapeutic development. Current studies focus on elucidating USP48’s role in chromatin remodeling, disease pathogenesis, and its potential as a biomarker or therapeutic target, underscoring its relevance in both basic and translational research.
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