| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP44 |
| Uniprot No | Q9H0E7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-712 aa |
| 活性数据 | MLAMDTCKHV GQLQLAQDHS SLNPQKWHCV DCNTTESIWA CLSCSHVACG RYIEEHALKH FQESSHPVAL EVNEMYVFCY LCDDYVLNDN TTGDLKLLRR TLSAIKSQNY HCTTRSGRFL RSMGTGDDSY FLHDGAQSLL QSEDQLYTAL WHRRRILMGK IFRTWFEQSP IGRKKQEEPF QEKIVVKREV KKRRQELEYQ VKAELESMPP RKSLRLQGLA QSTIIEIVSV QVPAQTPASP AKDKVLSTSE NEISQKVSDS SVKRRPIVTP GVTGLRNLGN TCYMNSVLQV LSHLLIFRQC FLKLDLNQWL AMTASEKTRS CKHPPVTDTV VYQMNECQEK DTGFVCSRQS SLSSGLSGGA SKGRKMELIQ PKEPTSQYIS LCHELHTLFQ VMWSGKWALV SPFAMLHSVW RLIPAFRGYA QQDAQEFLCE LLDKIQRELE TTGTSLPALI PTSQRKLIKQ VLNVVNNIFH GQLLSQVTCL ACDNKSNTIE PFWDLSLEFP ERYQCSGKDI ASQPCLVTEM LAKFTETEAL EGKIYVCDQC NSKRRRFSSK PVVLTEAQKQ LMICHLPQVL RLHLKRFRWS GRNNREKIGV HVGFEEILNM EPYCCRETLK SLRPECFIYD LSAVVMHHGK GFGSGHYTAY CYNSEGGFWV HCNDSKLSMC TMDEVCKAQA YILFYTQRVT ENGHSKLLPP ELLLGSQHPN EDADTSSNEI LS |
| 分子量 | 81.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人USP44蛋白的文献示例及摘要概述(部分信息基于领域内典型研究方向推测，建议通过文献数据库核实具体内容)：
---
1. **标题**: *"USP44 is required for chromosome alignment via deubiquitinating activity in mouse oocyte meiosis"*
**作者**: Zhang M, Zhang J, et al.
**摘要**: 研究利用重组人USP44蛋白，验证其在哺乳动物卵母细胞减数分裂中的去泛素化功能。结果显示，USP44通过调控APC/C复合体底物的稳定性，保障染色体正确排列，重组蛋白实验表明其直接作用于细胞周期关键蛋白的泛素链修剪。
---
2. **标题**: *"Structural basis of USP44-mediated deubiquitination and spindle checkpoint regulation"*
**作者**: Barford D, Yu Z, et al.
**摘要**: 通过重组人USP44蛋白的晶体结构解析，揭示其底物识别与催化机制。该研究发现USP44通过独特结构域与纺锤体检查点蛋白Mad1结合，并证实其去泛素化活性对维持有丝分裂检查点完整性至关重要。
---
3. **标题**: *"USP44 antagonizes p53 nuclear exclusion by deubiquitinating PML"*
**作者**: Wang L, Chen C, et al.
**摘要**: 文章利用重组USP44蛋白进行体外泛素化实验，证明其通过去泛素化PML蛋白，阻止p53的核输出，从而增强DNA损伤应答。实验表明USP44的酶活缺失突变体会导致肿瘤细胞凋亡抵抗。
---
**备注**：上述文献标题与内容为示例性质，实际研究需以PubMed、Google Scholar等数据库检索为准(关键词：USP44. recombinant protein, deubiquitinase)。推荐查阅近五年高被引论文以获取最新机制研究。
**Background of Recombinant Human USP44 Protein**
Recombinant human USP44 (Ubiquitin-Specific Protease 44) is a genetically engineered protein belonging to the deubiquitinating enzyme (DUB) family, which regulates protein stability and function by removing ubiquitin moieties from target substrates. USP44 plays a critical role in cell cycle control, particularly during mitosis. It is implicated in the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring proper chromosome segregation by delaying anaphase until all chromosomes are correctly attached to the mitotic spindle. USP44 interacts with key SAC components, such as Mad2 and CDC20. to prevent premature cell cycle progression, thereby maintaining genomic stability.
Dysregulation of USP44 has been linked to chromosomal instability, a hallmark of cancers and developmental disorders. Beyond mitosis, USP44 also participates in DNA damage repair, inflammatory signaling, and transcriptional regulation, highlighting its multifaceted roles. The recombinant form of USP44 is commonly produced in vitro using expression systems like *E. coli* or mammalian cells, enabling studies on its enzymatic activity, structural properties, and interactions. Its purified form serves as a vital tool for investigating DUB-mediated pathways, drug discovery targeting ubiquitination-related diseases, and deciphering mechanisms underlying tumorigenesis or chemotherapy resistance. Research on recombinant USP44 continues to expand its potential as a therapeutic or diagnostic biomarker in precision medicine.
×
