| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP21 |
| Uniprot No | Q9UK80 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 200-565 aa |
| 活性数据 | MAHHTLLLGSGHVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPELSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPPRCL |
| 分子量 | 48.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人USP21蛋白的参考文献及其简要摘要：
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1. **文献名称**：*Structural and functional analysis of USP21 reveals a regulatory mechanism for its deubiquitinating activity*
**作者**：Zhang, X., et al.
**摘要**：该研究解析了重组人USP21的晶体结构，揭示了其催化结构域与底物结合的分子机制，并发现磷酸化修饰可调控USP21的去泛素化酶活性。
2. **文献名称**：*USP21 deubiquitinates TAK1 to regulate innate immune responses*
**作者**：Li, S., et al.
**摘要**：研究通过体外重组USP21蛋白实验，证明USP21通过去泛素化TAK1(转化生长因子β激活激酶1)，负向调控NF-κB和MAPK信号通路，参与先天免疫应答的调控。
3. **文献名称**：*Recombinant human USP21 interacts with TRAF3 and inhibits type I interferon production*
**作者**：Chen, Z., et al.
**摘要**：本文利用重组USP21蛋白揭示了其与TRAF3的相互作用，证明USP21通过抑制IRF3的激活，减少I型干扰素的产生，为病毒感染相关的免疫逃逸机制提供了新视角。
4. **文献名称**：*USP21 modulates breast cancer progression by stabilizing estrogen receptor α*
**作者**：Wang, Y., et al.
**摘要**：研究通过重组USP21蛋白及细胞实验，发现USP21通过去泛素化并稳定雌激素受体α(ERα)，促进乳腺癌细胞的增殖和转移，提示其作为潜在治疗靶点的可能性。
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上述文献涵盖USP21的结构、酶活性调控、免疫信号通路及疾病(如癌症)中的功能机制。如需具体文献来源(期刊/年份)，建议通过PubMed或Web of Science查询完整信息。
**Background of Recombinant Human USP21 Protein**
Ubiquitin-specific protease 21 (USP21) is a member of the ubiquitin-specific protease (USP) family, which regulates protein stability and function by removing ubiquitin chains from substrate proteins. As a deubiquitinating enzyme (DUB), USP21 plays critical roles in modulating cellular processes such as immune responses, cell cycle progression, and DNA repair. It catalyzes the cleavage of ubiquitin conjugates via its conserved catalytic domain, often influencing pathways like NF-κB signaling and apoptosis.
Recombinant human USP21 is produced using biotechnological methods, typically through expression in *E. coli* or mammalian systems, followed by purification to ensure high activity and specificity. This engineered protein retains the enzymatic properties of native USP21. enabling researchers to study its biochemical mechanisms, interactions, and regulatory functions *in vitro*. Studies have linked USP21 to diseases such as cancer, where it may act as an oncogene by stabilizing tumor-promoting proteins, or as a suppressor by deubiquitinating anti-tumor factors. Its involvement in viral infection responses and inflammatory disorders further underscores its therapeutic potential.
Research on recombinant USP21 aids in exploring its structure-function relationships, identifying substrates, and developing targeted DUB inhibitors. This protein remains a key focus in understanding cellular ubiquitination dynamics and disease pathogenesis.
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