| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USF2 |
| Uniprot No | Q15853 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346 aa |
| 活性数据 | MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ |
| 分子量 | 36.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人USF2蛋白的3篇参考文献示例，涵盖不同研究方向：
---
1. **文献名称**: *"Purification and functional analysis of recombinant human USF2: DNA-binding specificity and transcriptional activation"*
**作者**: A. Sirito et al.
**摘要**: 研究利用大肠杆菌系统表达了重组人USF2蛋白，并通过亲和层析纯化。通过电泳迁移率变动实验(EMSA)证实其特异性结合靶基因启动子的E-box序列(CACGTG)，并发现其转录激活功能依赖于C端结构域的磷酸化修饰。
2. **文献名称**: *"Structural characterization of recombinant human USF2 and its interaction with TFIIB"*
**作者**: K. Atchison, M. L. Gualtieri
**摘要**: 通过X射线晶体学解析了重组人USF2的碱性螺旋-环-螺旋(bHLH)结构域，揭示了其与DNA结合的分子机制。进一步实验表明，USF2可通过N端结构域直接结合转录因子TFIIB，协同调控基因表达。
3. **文献名称**: *"Role of recombinant USF2 in hepatic glucose metabolism: implications for type 2 diabetes"*
**作者**: Q. Wang et al.
**摘要**: 利用重组人USF2蛋白在小鼠肝细胞模型中验证其调控糖异生关键基因(如PEPCK)的作用。过表达USF2显著抑制高血糖状态下PEPCK启动子活性，提示其在胰岛素抵抗中的潜在治疗价值。
---
**备注**：以上文献为示例，实际引用时需根据具体研究内容检索PubMed或Web of Science获取真实文献信息。
Upstream stimulatory factor 2 (USF2) is a member of the USF family of transcription factors, which regulate gene expression by binding to E-box motifs (CANNTG) in promoter regions. Structurally, USF2 contains a conserved basic helix-loop-helix (bHLH) domain for DNA binding and a dimerization domain, allowing it to form homo- or heterodimers (e.g., with USF1) to modulate target gene activity. It functions broadly in cellular processes, including cell cycle regulation, metabolic homeostasis, and stress responses.
Recombinant human USF2 protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., His-tag). This engineered protein retains DNA-binding functionality, enabling in vitro studies on transcriptional mechanisms, protein-DNA interactions, and regulatory networks. Research highlights its role in liver metabolism (e.g., lipid synthesis), muscle differentiation, and immune regulation through target genes such as *TNF-α* and *PGC-1α*. Dysregulation of USF2 has been linked to metabolic disorders, cancer progression, and neurodegenerative diseases, underscoring its biomedical relevance. Its recombinant form is a critical tool for elucidating gene regulatory pathways and screening therapeutic targets.
×
