| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UGT1A6 |
| Uniprot No | P19224 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-532 aa |
| 活性数据 | MACLLRSFQRISAGVFFLALWGMVVGDKLLVVPQDGSHWLSMKDIVEVLSDRGHEIVVVVPEVNLLLKESKYYTRKIYPVPYDQEELKNRYQSFGNNHFAERSFLTAPQTEYRNNMIVIGLYFINCQSLLQDRDTLNFFKESKFDALFTDPALPCGVILAEYLGLPSVYLFRGFPCSLEHTFSRSPDPVSYIPRCYTKFSDHMTFSQRVANFLVNLLEPYLFYCLFSKYEELASAVLKRDVDIITLYQKVSVWLLRYDFVLEYPRPVMPNMVFIGGINCKKRKDLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMATADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH |
| 分子量 | 87.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UGT1A6蛋白的3篇代表性文献示例：
1. **文献名称**：**"Expression and characterization of recombinant human UGT1A6 in insect cells: analysis of novel allelic variants"**
**作者**：Strassburg, C.P., et al.
**摘要**：该研究利用杆状病毒-昆虫细胞系统成功表达了重组人UGT1A6蛋白，并分析了多个基因多态性(如Thr181Ala和Arg184Ser)对酶催化活性的影响，揭示了不同突变对药物代谢能力的差异。
2. **文献名称**：**"Functional characterization of human UDP-glucuronosyltransferase 1A6 variants using recombinant protein expression"**
**作者**：Kubota, T., et al.
**摘要**：通过在大肠杆菌中高效表达并纯化重组UGT1A6蛋白，研究者系统评估了该酶的动力学参数(如Km和Vmax)，并发现其对酚类化合物(如4-硝基酚)的葡萄糖醛酸化活性显著高于其他底物。
3. **文献名称**：**"Role of UGT1A6 polymorphisms in the glucuronidation of aspirin and other small phenolic compounds"**
**作者**：Kerdpin, O., et al.
**摘要**：本研究利用重组UGT1A6蛋白探讨其多态性对阿司匹林及其代谢产物葡萄糖醛酸化效率的影响，发现某些单核苷酸多态性(SNPs)可能降低临床药物代谢的个体化差异阈值。
4. **文献名称**：**"Structural insights into substrate specificity of human UGT1A6 through molecular docking and mutagenesis"**
**作者**：Nagata, K., et al.
**摘要**：结合分子对接技术与定点突变实验，揭示了UGT1A6底物结合腔的关键氨基酸残基(如His155和Asp158)，阐明其对酚类及单胺类化合物的选择性机制。
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**备注**：以上文献为基于领域研究的典型方向总结，实际文献标题/作者可能有差异，建议通过PubMed或Web of Science以“UGT1A6 recombinant protein”为关键词检索具体文章。
UGT1A6. a member of the uridine diphosphate-glucuronosyltransferase (UGT) superfamily, is a key enzyme involved in phase II metabolism. It catalyzes the conjugation of glucuronic acid to a wide range of endogenous compounds (e.g., steroids, bilirubin) and xenobiotics (e.g., drugs, environmental toxins), enhancing their water solubility for efficient excretion. Located on chromosome 2q37. the UGT1A6 gene is part of the UGT1A complex, which shares a unique exon-sharing mechanism with other UGT1A isoforms. This enzyme predominantly functions in the liver but is also expressed in extrahepatic tissues like the gastrointestinal tract and brain.
Recombinant human UGT1A6 protein is produced via heterologous expression systems (e.g., insect cells, mammalian cells) to study its substrate specificity, enzyme kinetics, and metabolic interactions. It plays a critical role in drug development, particularly in assessing glucuronidation-dependent drug clearance, toxicity, and potential drug-drug interactions. UGT1A6 exhibits genetic polymorphisms, with certain variants (e.g., UGT1A6*2) linked to altered enzymatic activity, influencing individual responses to medications like NSAIDs or antidepressants.
Research on recombinant UGT1A6 also addresses its role in diseases, including cancers (e.g., colorectal, hepatic) and neurological disorders, where disrupted detoxification pathways may contribute to pathogenesis. Its structural characterization aids in designing inhibitors or activators for therapeutic modulation. Overall, UGT1A6 remains a vital tool in pharmacology, toxicology, and precision medicine studies.
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