| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD3G |
| Uniprot No | P09693 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-116aa |
| 氨基酸序列 | QSIKGNHLVKVYDYQEDGSVLLTCDAEAKNITWFKDGKMIGFLTEDKKKWNLGSNAKDPRGMYQCKGSQNKSKPLQVYYRMCQNCIELNAATIS |
| 预测分子量 | 18.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD3G重组蛋白的模拟参考文献示例(内容基于研究领域常见方向，非真实文献)：
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1. **文献名称**：Structural and Functional Characterization of Recombinant CD3γ Protein in T Cell Signaling
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌系统表达并纯化了重组CD3G蛋白，利用X射线晶体学解析其胞内结构域的三维结构，揭示了其与CD3ε亚基的关键相互作用界面，为T细胞受体信号传导机制提供了结构基础。
2. **文献名称**：Expression and Purification of CD3γ in Mammalian Cells for Immunotherapy Development
**作者**：Lee H, et al.
**摘要**：作者通过哺乳动物细胞系(如HEK293)成功表达功能性CD3G重组蛋白，优化纯化工艺后证明其可增强嵌合抗原受体(CAR-T)细胞的激活效率，为肿瘤免疫治疗提供了新策略。
3. **文献名称**：CD3γ Deficiency Rescue by Recombinant Protein Delivery in Zebrafish Models
**作者**：García-Rodríguez MC, et al.
**摘要**：通过斑马鱼模型研究CD3G缺失对T细胞发育的影响，发现外源性重组CD3G蛋白可部分恢复T细胞功能，提示其在先天性免疫缺陷疾病中的潜在治疗价值。
4. **文献名称**：Interaction Mapping of CD3γ with TCR Subunits Using Recombinant Proteins
**作者**：Wang Q, et al.
**摘要**：利用重组CD3G及TCR其他亚基蛋白进行体外结合实验，结合质谱分析鉴定出CD3G与TCRα/β链的磷酸化依赖性互作网络，揭示了其在信号转导中的动态调控机制。
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注：以上文献为模拟内容，实际引用需查询PubMed、Web of Science等数据库获取真实文献。
CD3G (CD3 gamma) recombinant protein is a key component in the study of T-cell receptor (TCR) signaling and immune regulation. CD3G is one of the subunits of the CD3 complex, a multimeric protein complex associated with the TCR on the surface of T lymphocytes. The CD3 complex consists of γ, δ, ε, and ζ subunits, which are essential for TCR assembly, surface expression, and signal transduction during antigen recognition. CD3G, encoded by the CD3G gene in humans (chromosome 11), is a transmembrane protein with an extracellular immunoglobulin-like domain, a transmembrane region, and a short intracellular tail containing immunoreceptor tyrosine-based activation motifs (ITAMs). These ITAMs play a critical role in propagating activation signals upon TCR engagement with antigen-presenting cells.
Recombinant CD3G protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the protein for research and therapeutic applications. Its production often involves codon optimization, fusion tags (e.g., His-tag), or modifications to enhance solubility and stability. Researchers utilize CD3G recombinant protein to investigate TCR-CD3 complex assembly, T-cell activation mechanisms, and immune dysregulation in diseases like autoimmune disorders, immunodeficiency syndromes, and cancers. It is also employed in developing immunotherapies, such as bispecific antibodies or chimeric antigen receptor (CAR) T-cell therapies, where modulating TCR signaling is crucial.
Despite its utility, challenges remain in maintaining native conformational integrity and post-translational modifications during recombinant production. Ongoing studies focus on optimizing expression systems and structural characterization to advance its clinical relevance in targeted immune modulation.
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