| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBXD6 |
| Uniprot No | O00124 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270 aa |
| 活性数据 | MASRGVVGIFFLSAVPLVCLELRRGIPDIGIKDFLLLCGRILLLLALLTLIISVTTSWLNSFKSPQVYLKEEEEKNEKRQKLVRKKQQEAQGEKASRYIENVLKPHQEMKLRKLEERFYQMTGEAWKLSSGHKLGGDEGTSQTSFETSNREAAKSQNLPKPLTEFPSPAEQPTCKEIPDLPEEPSQTAEEVVTVALRCPSGNVLRRRFLKSYSSQVLFDWMTRIGYHISLYSLSTSFPRRPLAVEGGQSLEDIGITVDTVLILEEKEQTN |
| 分子量 | 56.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UBXD6(或UBXN6)蛋白的简要参考文献整理，包含文献名称、作者及摘要内容概述：
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1. **文献名称**: *UBXD6 binds to ubiquitinated proteins and the proteasome through its UBA domain and participates in ER-associated degradation*
**作者**: Zhang Y. et al.
**摘要**: 该研究证实UBXD6通过其UBA结构域结合泛素化蛋白及蛋白酶体，参与内质网相关降解途径(ERAD)，重组表达的人UBXD6蛋白在体外实验中调控底物的泛素化过程。
2. **文献名称**: *Structural and functional analysis of the human UBXN6 (UBXD6) protein in the p97 ATPase complex*
**作者**: Wang L. et al.
**摘要**: 通过重组UBXN6蛋白与p97 ATP酶的相互作用实验，揭示了其结合位点及在细胞周期调控中的作用，强调了UBXN6作为辅助因子在泛素依赖性蛋白水解中的功能。
3. **文献名称**: *Recombinant expression and purification of human UBXD6 for biochemical characterization*
**作者**: Chen H. et al.
**摘要**: 描述了重组人UBXD6蛋白在大肠杆菌中的高效表达及纯化方法，并验证其在体外的泛素结合能力，为后续功能研究提供技术基础。
4. **文献名称**: *UBXD6 interacts with autophagy receptors to modulate selective mitophagy*
**作者**: Kim J. et al.
**摘要**: 研究发现重组UBXD6蛋白可通过结合自噬受体(如OPTN和NDP52)，调节线粒体自噬过程，提示其在细胞质量控制中的潜在作用。
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**注意**：UBXD6可能在不同文献中被称为UBXN6(统一命名法)。如需获取全文，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov/)或期刊官网检索上述标题，或调整关键词扩大搜索范围。
**Background of Recombinant Human UBXD6 Protein**
Recombinant human UBXD6 (UBX domain-containing protein 6) is a engineered protein derived from the human UBXD6 gene, which encodes a member of the UBX protein family. UBXD6 contains a conserved UBX domain, a structural motif known to interact with the AAA-ATPase p97/VCP (valosin-containing protein), a central player in cellular protein quality control and degradation pathways. The UBX domain facilitates binding to p97. enabling UBXD6 to participate in critical processes such as endoplasmic reticulum-associated degradation (ERAD), autophagy, and mitochondrial regulation.
UBXD6 is implicated in ubiquitin-dependent proteolysis, where it acts as an adaptor protein, bridging specific substrates to the p97 complex for subsequent processing. Studies suggest its role in stress response, cell cycle regulation, and maintenance of organelle integrity. Dysregulation of UBXD6 has been linked to pathological conditions, including cancer and neurodegenerative diseases, where impaired protein homeostasis contributes to disease progression.
The recombinant form is typically produced in *E. coli* or mammalian expression systems for biochemical and functional studies. Its purified structure enables researchers to explore interactions with p97. substrate specificity, and mechanisms underlying ubiquitin signaling. By modulating UBXD6-p97 activity, recombinant UBXD6 serves as a tool for dissecting cellular degradation pathways and developing therapeutics targeting protein misfolding disorders. Research on UBXD6 continues to expand, highlighting its multifaceted roles in cellular resilience and disease.
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