| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBXD3 |
| Uniprot No | Q96LJ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-280 aa |
| 活性数据 | MATEAPVNIAPPECSTVVSTAVDSLIWQPNSLNMHMIRPKSAKGRTRPSLQKSQGVEVCAHHIPSPPPAIPYELPSSQKPGACAPKSPNQGASDEIPELQQQVPTGASSSLNKYPVLPSINRKNLEEEAVETVAKKASSLQLSSIRALYQDETGTMKTSEEDSRARACAVERKFIVRTKKQGSSRAGNLEEPSDQEPRLLLAVRSPTGQRFVRHFRPTDDLQTIVAVAEQKNKTSYRHCSIETMEVPRRRFSDLTKSLQECRIPHKSVLGISLEDGEGWP |
| 分子量 | 57.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UBXD3蛋白的3篇参考文献，涵盖功能、相互作用及疾病关联研究：
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1. **文献名称**: *UBXD3 interacts with the AAA-ATPase p97 and plays a role in the ER-associated degradation pathway*
**作者**: Yang Y, et al.
**摘要**: 本研究揭示了UBXD3作为p97/VCP ATP酶的辅助因子，参与内质网相关降解(ERAD)途径。通过重组人UBXD3蛋白的表达和体外结合实验，证实其UBX结构域与p97的N端结构域相互作用，并促进泛素化底物的蛋白酶体降解。
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2. **文献名称**: *Structural characterization of the UBXD3 UBX domain and its interaction with ubiquitin regulatory complexes*
**作者**: Liu H, et al.
**摘要**: 该研究解析了UBXD3的UBX结构域晶体结构，发现其通过保守的疏水界面与泛素结合蛋白相互作用。重组UBXD3在体外实验中显示出调节泛素链组装的能力，表明其在细胞泛素信号传导中的潜在调控作用。
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3. **文献名称**: *UBXD3 upregulation in breast cancer correlates with tumor progression and poor prognosis*
**作者**: Wang L, et al.
**摘要**: 通过分析乳腺癌组织样本，研究者发现UBXD3表达水平与肿瘤恶性程度呈正相关。实验中使用重组UBXD3蛋白验证其促进癌细胞侵袭的分子机制，提示UBXD3可能通过调控基质金属蛋白酶(MMP)通路驱动癌症转移。
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*注：以上文献为基于常见研究的虚拟示例，实际文献需通过学术数据库查询确认。*
Recombinant human UBXD3 (UBX domain-containing protein 3) is a engineered protein derived from the endogenous UBXD3. which functions as a cofactor in ubiquitin-dependent cellular processes. UBXD3 contains a conserved UBX domain, enabling interaction with p97/VCP, an ATPase critical for protein homeostasis. It also harbors a UBA domain, facilitating binding to ubiquitinated substrates. UBXD3 is implicated in endoplasmic reticulum-associated degradation (ERAD) and mitochondrial quality control by mediating the extraction and processing of misfolded proteins or damaged organelles via the ubiquitin-proteasome system. Studies suggest its role in modulating stress responses, cell cycle regulation, and apoptosis. Dysregulation of UBXD3 has been linked to neurodegenerative diseases and cancers, attributed to its involvement in protein aggregation clearance and genomic stability. Recombinant UBXD3 is typically produced in prokaryotic or eukaryotic expression systems, enabling biochemical studies, structural analyses, and screening for therapeutic compounds targeting p97-associated pathways. Its recombinant form retains functional domains, making it valuable for dissecting molecular mechanisms in ubiquitination-related pathologies. Current research focuses on its regulatory crosstalk with autophagy pathways and potential applications in drug development for disorders linked to impaired protein degradation.
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