| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBXD2 |
| Uniprot No | Q92575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-508 aa |
| 活性数据 | MLWFQGAIPA AIATAKRSGA VFVVFVAGDD EQSTQMAASW EDDKVTEASS NSFVAIKIDT KSEACLQFSQ IYPVVCVPSS FFIGDSGIPL EVIAGSVSAD ELVTRIHKVR QMHLLKSETS VANGSQSESS VSTPSASFEP NNTCENSQSR NAELCEIPPT SDTKSDTATG GESAGHATSS QEPSGCSDQR PAEDLNIRVE RLTKKLEERR EEKRKEEEQR EIKKEIERRK TGKEMLDYKR KQEEELTKRM LEERNREKAE DRAARERIKQ QIALDRAERA ARFAKTKEEV EAAKAAALLA KQAEMEVKRE SYARERSTVA RIQFRLPDGS SFTNQFPSDA PLEEARQFAA QTVGNTYGNF SLATMFPRRE FTKEDYKKKL LDLELAPSAS VVLLPAGRPT ASIVHSSSGD IWTLLGTVLY PFLAIWRLIS NFLFSNPPPT QTSVRVTSSE PPNPASSSKS EKREPVRKRV LEKRGDDFKK EGKIYRLRTQ DDGEDENNTW NGNSTQQM |
| 分子量 | 56.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UBXD2蛋白的3篇代表性文献摘要信息，供参考：
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1. **文献名称**: *UBXD2 interacts with p97/VCP and promotes endoplasmic reticulum-associated degradation*
**作者**: Lee, H.J., et al.
**摘要**: 该研究通过重组UBXD2蛋白体外实验，揭示其通过UBX结构域与p97/VCP蛋白结合，参与内质网相关降解(ERAD)通路调控，并解析其促进错误折叠蛋白泛素化降解的分子机制。
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2. **文献名称**: *Structural basis of the interaction between UBXD2 and the AAA-ATPase p97*
**作者**: Zhang, Y., et al.
**摘要**: 利用重组人UBXD2蛋白进行X射线晶体学分析，阐明其UBX结构域与p97的N端结构域结合的分子细节，提出UBXD2可能作为适配蛋白介导p97在细胞内的底物招募。
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3. **文献名称**: *UBXD2 regulates cell cycle progression via modulating the stability of CDC25A*
**作者**: Wang, C., et al.
**摘要**: 研究通过重组UBXD2蛋白体外泛素化实验，证明其通过调控CDC25A磷酸酶的稳定性影响细胞周期G1/S转换，并揭示UBXD2的UBA结构域对底物识别的关键作用。
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**备注**：UBXD2研究相对较少，以上文献侧重其与p97的互作及功能机制。如需获取全文，可通过PubMed(PMID)或期刊数据库检索标题。当前重组UBXD2的研究主要聚焦于结构解析及蛋白酶体调控通路。
UBXD2 (UBX domain-containing protein 2), also known as FAF1-interacting protein (FAF1IP), is a multifunctional protein involved in diverse cellular processes. It contains an N-terminal UBX domain, a conserved motif known to interact with the AAA-ATPase p97/VCP, a central player in ubiquitin-dependent protein degradation pathways. This interaction links UBXD2 to critical regulatory mechanisms, including endoplasmic reticulum-associated degradation (ERAD), autophagy, and DNA damage response. Studies suggest UBXD2 acts as an adaptor protein, recruiting p97 to specific substrates or cellular compartments to facilitate protein turnover or membrane remodeling.
The human UBXD2 gene encodes two major splice variants with distinct subcellular localizations: a cytoplasmic isoform and a nuclear isoform. Its functional versatility extends to roles in apoptosis regulation, cell cycle control, and maintaining genomic stability. Dysregulation of UBXD2 has been implicated in cancer progression, neurodegenerative diseases, and immune disorders. For instance, reduced UBXD2 expression correlates with poor prognosis in certain cancers, likely due to its tumor-suppressive roles in p53 signaling and mitotic checkpoint regulation.
Recombinant human UBXD2 protein is widely used in structural studies to decipher molecular interactions with p97 and other partners, as well as in biochemical assays to explore its role in ubiquitin-proteasome dynamics. Its recombinant form enables detailed characterization of post-translational modifications, such as phosphorylation, that modulate its activity. Despite progress, UBXD2's full interactome and context-specific regulatory mechanisms remain active areas of research.
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