| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBTD1 |
| Uniprot No | Q9HAC8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227 aa |
| 活性数据 | MGNCVGRQRR ERPAAPGHPR KRAGRNEPLK KERLKWKSDY PMTDGQLRSK RDEFWDTAPA FEGRKEIWDA LKAAAYAAEA NDHELAQAIL DGASITLPHG TLCECYDELG NRYQLPIYCL SPPVNLLLEH TEEESLEPPE PPPSVRREFP LKVRLSTGKD VRLSASLPDT VGQLKRQLHA QEGIEPSWQR WFFSGKLLTD RTRLQETKIQ KDFVIQVIIN QPPPPQD |
| 分子量 | 25.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UBTD1蛋白的参考文献示例(部分内容为模拟，实际文献需通过数据库核实)：
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1. **文献名称**: *UBTD1 regulates proteasomal degradation via UBA ubiquitin-binding domains*
**作者**: Zhang L, et al.
**摘要**: 本研究利用重组人UBTD1蛋白进行体外泛素化分析，发现UBTD1通过其UBA结构域结合泛素化底物，调控26S蛋白酶体依赖的蛋白降解通路，并验证了其对细胞周期蛋白稳定性的影响。
2. **文献名称**: *Structural basis of UBTD1 recognition by E3 ubiquitin ligases*
**作者**: Chen Y, et al.
**摘要**: 通过X射线晶体学解析重组UBTD1蛋白的结构，揭示其与E3泛素连接酶(如MDM2)的相互作用界面，阐明UBTD1在p53泛素化及肿瘤抑制中的分子机制。
3. **文献名称**: *UBTD1 promotes cell proliferation in non-small cell lung cancer through Wnt/β-catenin signaling*
**作者**: Wang H, et al.
**摘要**: 利用重组UBTD1蛋白处理肺癌细胞，证明其通过激活Wnt/β-catenin信号通路促进肿瘤细胞增殖，提示UBTD1可能成为肺癌治疗的新靶点。
4. **文献名称**: *Development of a recombinant UBTD1 expression system for high-throughput screening of ubiquitination inhibitors*
**作者**: Müller S, et al.
**摘要**: 建立基于重组UBTD1蛋白的高通量筛选平台，用于鉴定调控泛素化-蛋白酶体系统的抑制剂，为神经退行性疾病药物开发提供工具。
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**注意**：以上文献及摘要内容为模拟生成，实际研究中需在**PubMed/Google Scholar**等平台以关键词“UBTD1”、“recombinant UBTD1”、“ubiquitin”检索最新文献。可重点关注细胞增殖、泛素-蛋白酶体系统、癌症及结构生物学相关领域。
**Background of Recombinant Human UBTD1 Protein**
Ubiquitin domain-containing protein 1 (UBTD1) is a conserved eukaryotic protein implicated in regulating ubiquitin-dependent cellular processes. It contains a characteristic ubiquitin-associated (UBA) domain, enabling interactions with ubiquitinated substrates and components of the ubiquitin-proteasome system (UPS). UBTD1 modulates protein stability, localization, and degradation by acting as an adaptor or scaffold for E3 ubiquitin ligases, such as the Cullin-RING ligase complexes, influencing pathways like cell cycle progression, DNA damage response, and apoptosis.
Recombinant human UBTD1 protein is generated via heterologous expression systems (e.g., *E. coli* or mammalian cells), ensuring high purity and bioactivity for functional studies. Structural and biochemical analyses reveal its role in binding polyubiquitin chains and facilitating substrate recognition by the 26S proteasome. UBTD1 also interacts with key regulators like p53 and cyclin-dependent kinases (CDKs), suggesting involvement in tumor suppression and oncogenesis. Dysregulation of UBTD1 has been linked to cancers and neurodegenerative diseases, highlighting its therapeutic potential.
As a research tool, recombinant UBTD1 aids in deciphering UPS mechanisms, ubiquitin signaling dynamics, and disease-related molecular pathways. Its study offers insights into targeted therapies for conditions driven by proteostasis imbalance.
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