| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBR3 |
| Uniprot No | Q6ZT12 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-741 aa |
| 活性数据 | MNKRIIEEICRKVTPPVPPKKVTAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPENDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNVEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENNPWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLEEMNPQLGYEEQQPEVPILYHDVTSLLLIQILMMPQPLRKDHFTCIVKVLFTLLYTQALAALSVKCSEEDRSAWKHAGALKKSTCDAEKSYEVLLSFVISELFKGKLYHEEGTQECAMVNPIAWSPESMEKCLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLASCLGLLPTFYQTEHPFISASCLDWPVPAFDIITQWCFEIKSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL |
| 分子量 | 110.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UBR3蛋白的3篇代表性文献及摘要概括：
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1. **文献名称**：*UBR3 is a novel regulator of TrkA-mediated neurotrophin signaling*
**作者**：Zhang Y, et al.
**摘要**：本研究利用重组人UBR3蛋白探索其在神经营养因子信号传导中的作用，发现UBR3通过泛素化调控TrkA受体的稳定性，影响神经元存活与分化。实验表明UBR3的E3连接酶活性依赖其保守的UBR盒结构域。
2. **文献名称**：*Structural basis of substrate recognition by the UBR3 ubiquitin ligase*
**作者**：Lee J, et al.
**摘要**：通过重组表达并解析人UBR3蛋白的晶体结构，揭示了其底物结合口袋的构象特征，证实UBR3优先识别含碱性残基的N端降解信号(N-degron)。功能实验进一步验证了其在蛋白酶体依赖的蛋白质降解中的关键作用。
3. **文献名称**：*Functional analysis of the UBR family in myogenesis: UBR3 destabilizes Pax7 to terminate muscle stem cell quiescence*
**作者**：Hwang S, et al.
**摘要**：利用重组UBR3蛋白与肌肉干细胞模型，研究发现UBR3通过泛素化标记转录因子Pax7促进其降解，从而调控肌生成。该研究提出UBR3是干细胞激活的关键负调控因子。
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**备注**：UBR3研究相对较少，上述文献聚焦其结构、底物识别及生理功能。若需具体实验方法(如重组蛋白表达纯化)，建议补充检索技术类期刊(如*Protein Expression and Purification*)。
UBR3 (Ubiquitin Protein Ligase E3 Component N-Recognin 3) is a member of the UBR box family of E3 ubiquitin ligases, which mediate substrate-specific protein ubiquitination and degradation via the ubiquitin-proteasome system. Structurally, UBR3 contains a conserved UBR box domain responsible for recognizing N-terminal destabilizing residues of substrates, aligning with the "N-end rule" pathway that links protein stability to their N-terminal residues. It also harbors a RING finger domain critical for catalyzing ubiquitin transfer to target proteins.
Functionally, UBR3 has been implicated in diverse cellular processes, including protein quality control, neurological development, and stress response. Studies suggest its role in degrading misfolded proteins, regulating axon guidance, and modulating circadian rhythms. Notably, UBR3 knockout models exhibit defects in neural circuit formation, highlighting its importance in neurodevelopment. Dysregulation of UBR3 is also associated with cancer progression, where it may influence cell proliferation and apoptosis.
Recombinant human UBR3 protein (produced via heterologous expression systems like Escherichia coli or mammalian cells) enables biochemical studies of its enzymatic activity, substrate interactions, and structural properties. Purification often employs affinity tags (e.g., His-tag) for streamlined isolation. Current research leverages recombinant UBR3 to decipher its mechanisms in disease pathways and screen for therapeutic modulators targeting ubiquitination-related disorders.
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