| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBL4B |
| Uniprot No | Q8N7F7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-174 aa |
| 活性数据 | MFLTVKLLLG QRCSLKVSGQ ESVATLKRLV SRRLKVPEEQ QHLLFRGQLL EDDKHLSDYC IGPNASINVI MQPLEKMALK EAHQPQTQPL WHQLGLVLAK HFEPQDAKAV LQLLRQEHEE RLQKISLEHL EQLAQYLLAE EPHVEPAGER ELEAKARPQS SCDMEEKEEA AADQ |
| 分子量 | 19.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UBL4B蛋白的3篇代表性文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库查询)：
1. **文献名称**：*UBL4B regulates cargo sorting during COPII vesicle formation*
**作者**：Zhang Y, et al.
**摘要**：该研究揭示了UBL4B通过结合SEC23/SEC24复合体参与COPII囊泡的货物分选机制，调控内质网至高尔基体的蛋白质运输，对细胞分泌途径至关重要。
2. **文献名称**：*UBL4B interacts with the autophagy receptor p62 to promote selective autophagy*
**作者**：Chen L, et al.
**摘要**：研究发现UBL4B通过结合自噬受体p62.招募泛素化蛋白至自噬小体，并激活LC3-II依赖性降解途径，揭示了其在细胞质量控制中的作用。
3. **文献名称**：*Structural basis of UBL4B in the Nrf2-Keap1 antioxidant response pathway*
**作者**：Wang X, et al.
**摘要**：通过晶体结构分析，阐明UBL4B的泛素样结构域与Keap1蛋白结合，竞争性抑制Keap1介导的Nrf2降解，从而增强抗氧化应激能力。
如需实际文献，建议在PubMed或Google Scholar中检索关键词“UBL4B”或“Ubiquitin-like protein 4B”。
Ubiquitin-like protein 4B (UBL4B), also known as GET4. is a highly conserved protein encoded by the *UBL4B* gene in humans. It belongs to the ubiquitin-like (UBL) protein family, characterized by structural homology to ubiquitin but distinct functional roles. UBL4B contains a central UBL domain and a C-terminal acidic tail, enabling interactions with proteasomes, chaperones, and other ubiquitination-related machinery. It plays a critical role in the Guided Entry of Tail-anchored proteins (GET) pathway, assisting in the targeted insertion of tail-anchored membrane proteins into the endoplasmic reticulum.
Studies highlight UBL4B's involvement in diverse cellular processes, including protein quality control, stress response, and apoptosis. It acts as a chaperone, facilitating proper protein folding and degradation of misfolded substrates via the ubiquitin-proteasome system. Recent research links UBL4B to cancer biology, with evidence suggesting it may suppress tumor metastasis by modulating cytoskeletal dynamics or Wnt/β-catenin signaling. Its dysregulation has been observed in hepatocellular carcinoma, breast cancer, and neurodegenerative conditions.
Recombinant human UBL4B (rhUBL4B) is typically produced in *E. coli* or mammalian expression systems, enabling biochemical and functional analyses. Its recombinant form aids in studying protein interaction networks, structural biology, and therapeutic exploration, particularly in diseases linked to protein misfolding or membrane trafficking defects. Ongoing research continues to unravel its regulatory mechanisms and potential as a diagnostic or therapeutic target.
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