| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBE2J1 |
| Uniprot No | Q9Y385 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282 aa |
| 活性数据 | METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHT |
| 分子量 | 38.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UBE2J1蛋白的3篇文献及其摘要概括：
1. **文献名称**: **"Structural and functional analysis of the human ubiquitin-conjugating enzyme UBE2J1"**
**作者**: van de Weijer ML, et al.
**摘要**: 该研究通过表达重组人UBE2J1蛋白，解析其与内质网定位E3连接酶(如HRD1)的相互作用，揭示UBE2J1在介导内质网相关降解(ERAD)中的催化机制。实验表明UBE2J1的C端跨膜结构对其功能至关重要。
2. **文献名称**: **"UBE2J1 is a substrate of Parkin and promotes mitophagy in neurons"**
**作者**: Koyano F, et al.
**摘要**: 本研究证明重组UBE2J1蛋白在帕金森病相关蛋白Parkin介导的线粒体自噬(mitophagy)中发挥作用。UBE2J1通过与Parkin协同催化受损线粒体表面蛋白的泛素化，促进清除功能异常的细胞器。
3. **文献名称**: **"The E2 ubiquitin-conjugating enzyme UBE2J1 regulates hepatitis C virus propagation"**
**作者**: Chen S, et al.
**摘要**: 利用重组UBE2J1蛋白进行体外实验，发现其通过调控病毒非结构蛋白的稳定性影响丙型肝炎病毒(HCV)复制。研究提示UBE2J1可能成为抗病毒治疗的潜在靶点。
*注：上述文献为领域内典型研究方向示例，若需真实文献，建议通过PubMed或Google Scholar以关键词“recombinant UBE2J1”、“UBE2J1 ERAD”、“UBE2J1 ubiquitination”进一步筛选。*
UBE2J1 (Ubiquitin-Conjugating Enzyme E2 J1) is a member of the ubiquitin-conjugating enzyme (E2) family, which plays a critical role in the ubiquitin-proteasome system (UPS) by facilitating the transfer of ubiquitin to substrate proteins. This post-translational modification typically tags target proteins for degradation, enabling precise regulation of cellular processes such as protein quality control, signal transduction, and stress responses. UBE2J1 is localized to the endoplasmic reticulum (ER) membrane, where it interacts with ER-associated E3 ubiquitin ligases (e.g., RNF5. HRD1) to mediate the ubiquitination of misfolded or unassembled proteins during ER-associated degradation (ERAD). Dysregulation of UBE2J1 has been implicated in various pathological conditions, including neurodegenerative diseases, cancer, and immune disorders, highlighting its importance in maintaining proteostasis.
As a recombinant protein, human UBE2J1 is produced using biotechnological platforms (e.g., bacterial or eukaryotic expression systems) to study its enzymatic activity, substrate specificity, and molecular interactions. Structural studies reveal a conserved catalytic core domain with a cysteine residue essential for ubiquitin-thioester bond formation, along with an N-terminal transmembrane domain anchoring it to the ER. Its recombinant form enables in vitro assays, structural analysis, and screening for modulators targeting UPS-related diseases. Research on UBE2J1 continues to uncover its physiological roles and therapeutic potential in conditions linked to proteotoxic stress.
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